Coprinopsis cinerea Uses Laccase Lcc9 as a Defense Strategy To Eliminate Oxidative Stress during Fungal-Fungal Interactions
Coprinopsis cinerea Uses Laccase Lcc9 as a Defense Strategy To Eliminate Oxidative Stress during Fungal-Fungal Interactions
复制标题
灰霉鬼伞 (Coprinopsis cinerea) 使用漆酶 Lcc9 作为防御策略来消除真菌与真菌相互作用期间的氧化应激
DOI：
10.1128/aem.01760-21
复制
发表时间：
2022-01-01
影响因子：
4.4
通讯作者：
Kuees, Ursula
中科院分区：
文献类型：
--
作者：
Liu, Juanjuan;Peng, Can;Kuees, Ursula
通常，漆酶在真菌共培养过程中被触发过度表达。这些活化漆酶在共培养过程中的功能尚未阐明。之前，我们报道了贡罗氏菌 sp。 w5 (w5)（毛霉菌门、毛霉菌门）特异性触发漆酶 Lcc9 在鸡冠草（担子菌门、伞形孢菌门）中过度表达。为了系统地分析过表达的漆酶在真菌相互作用过程中的功能，选择初始 Lcc9 过表达之前和之后的灰霉菌丝体进行转录组分析。结果表明，伴随着果糖作为碳水化合物底物的特异性利用，来自 w5 分泌的拮抗化合物的氧化应激似乎是灰霉病菌漆酶产生的关键信号。随着漆酶产量的增加，灰霉野生型菌株中活性氧（ROS）的减少，然后 lcc9 转录和漆酶活性停止。相比之下，在 kc9 沉默的 C. cinerea 菌株的整个共培养过程中，观察到 H2O2 含量和菌丝 ROS 水平增加。此外，kc9 沉默减慢了 C. cinerea 菌丝的生长，影响了菌丝形态，并减少了共培养中的无性孢子形成。我们的结果表明，细胞内ROS作为信号分子刺激灰霉病菌的防御反应，表达氧化应激反应调节因子Skn7和各种解毒蛋白。 Lcc9 在与 w5 的种间相互作用过程中参与消除氧化应激的防御策略。 重要性 众所周知，在种间真菌相互作用过程中漆酶的过量产生。然而，上调漆酶的确切作用仍未得到充分探索。基于灰霉病菌的比较转录组分析和漆酶 Lcc9 的基因沉默，我们在此表明氧化应激源自贡罗氏菌 (Gongronella sp.) 分泌的拮抗化合物。 w5 是灰霉鬼伞 (Coprinopsis cinerea) 漆酶 Lcc9 生产的关键信号。细胞内ROS作为信号分子刺激C. dnerea的防御反应，表达氧化应激反应调节因子Skn7和各种解毒蛋白。最终，Lcc9 参与防御策略，以消除氧化应激并在与贡罗氏菌属物种间相互作用期间帮助细胞生长和发育。 w5。这些发现加深了我们对其自然种群和群落中真菌相互作用的理解。
Frequently, laccases are triggered during fungal cocultivation for overexpression. The function of these activated laccases during coculture has not been clarified. Previously, we reported that Gongronella sp. w5 (w5) (Mucoromycota, Mucoromycetes) specifically triggered the laccase Lcc9 overexpression in Coprinopsis dnerea (Basidiomycota, Agaricomycetes). To systematically analyze the function of the overexpressed laccase during fungal interaction, C. cinerea mycelia before and after the initial Lcc9 overexpression were chosen for transcriptome analysis. Results showed that accompanied by specific utilization of fructose as carbohydrate substrate, oxidative stress derived from antagonistic compounds secreted by w5 appears to be a signal critical for laccase production in C. cinerea. A decrease in reactive oxygen species (ROS) in the C. cinerea wild-type strain followed the increase in laccase production, and then lcc9 transcription and laccase activity stopped. By comparison, increased H2O2 content and mycelial ROS levels were observed during the entire cocultivation in kc9 silenced C. cinerea strains. Moreover, kc9 silencing slowed down the C. cinerea mycelial growth, affected hyphal morphology, and decreased the asexual sporulation in coculture. Our results showed that intracellular ROS acted as signal molecules to stimulate defense responses by C. cinerea with the expression of oxidative stress response regulator Skn7 and various detoxification proteins. Lcc9 takes part in a defense strategy to eliminate oxidative stress during the interspecific interaction with w5.IMPORTANCE The overproduction of laccase during interspecific fungal interactions is well known. However, the exact role of the upregulated laccases remains underexplored. Based on comparative transcriptomic analysis of C. cinerea and gene silencing of laccase Lcc9, here we show that oxidative stress derived from antagonistic compounds secreted by Gongronella sp. w5 was a signal critical for laccase Lcc9 production in Coprinopsis cinerea. Intracellular ROS acted as signal molecules to stimulate defense responses by C. dnerea with the expression of oxidative stress response regulator Skn7 and various detoxification proteins. Ultimately, Lcc9 takes part in a defense strategy to eliminate oxidative stress and help cell growth and development during the interspecific interaction with Gongronella sp. w5. These findings deepened our understanding of fungal interactions in their natural population and communities.