EVALUATION OF THE DYE-PROTEIN TRACERS IN PATHO-PHYSIOLOGY OF THE BLOOD-BRAIN-BARRIER
EVALUATION OF THE DYE-PROTEIN TRACERS IN PATHO-PHYSIOLOGY OF THE BLOOD-BRAIN-BARRIER
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DOI：
10.1007/bf00691332
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发表时间：
1981-01-01
影响因子：
12.7
通讯作者：
SPATZ, M
中科院分区：
文献类型：
--
作者：
WOLMAN, M;KLATZO, I;SPATZ, M
荧光素钠和埃文斯蓝是研究血脑屏障紊乱的常用示踪剂，它们在血浆中各自的蛋白质结合能力、通过屏障的能力和从[大鼠]脑实质中消除的速度方面存在相当大的差异。在血浆中，相当一部分荧光素钠保持游离状态，表现得像微分子屏障示踪剂。埃文斯蓝几乎完全结合白蛋白赋予其蛋白质示踪剂的特性。随着示踪剂的外渗，荧光素钠相对很快被消除；Evans Blue在相当长的一段时间内仍存在于脑实质的细胞成分中，尽管示踪剂的蛋白质部分可能通过溶酶体消化从胶质细胞的细胞质中更快地去除。
Sodium fluorescein and Evans Blue, commonly used tracers in the study of blood-brain barrier disturbances, revealed considerable differences in their respective protein binding capacity in the plasma, passage through the barrier and in the rate of their elimination from [rat] brain parenchyma. In the plasma a considerable portion of the sodium fluorescein remains free and behaves like a micromolecular barrier tracer. Almost complete binding of the Evans Blue to albumin confers to it properties of a protein tracer. Following the extravasation of the tracers, the sodium fluorescein is relatively soon eliminated; Evans Blue remains in the cellular elements of the brain parenchyma for a considerable time, although the protein moiety of the tracer is removed sooner from the cytoplasm of glial cells, presumably by the lysosomal digestion.