A simple and small-scale hydroponic culture system for obtaining second-stage juveniles of the root-knot nematode
A simple and small-scale hydroponic culture system for obtaining second-stage juveniles of the root-knot nematode
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一种获得根结线虫二期幼虫的简易小型水培系统
DOI：
10.1007/s13355-015-0365-4
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发表时间：
2016
影响因子：
1.3
通讯作者：
Y. Umezaki
中科院分区：
文献类型：
--
作者：
T. Yoshiga;Y. Umezaki
我们报告使用一个简单的，小规模的水培系统，使用矮生品种的迷你番茄，获得第二阶段的青少年（J2）的南方根结线虫（Kofoid等白色）。该系统由番茄植物、市售的水培培养基、500-mL玻璃烧杯和连接到用于通气的气泵的通气石组成。结果表明，在至少20天内，每株植物每天可获得超过5000 J2 s的产量。在溶液培养开始后的11至21天之间获得每天约51，000 - 66，000 J2的最大产量。获得的J2的感染性与从卵块中收集的J2相当。
We report use of a simple, small-scale hydroponic culture system, using a dwarf variety of mini-tomato, to obtain second-stage juveniles (J2s) of Meloidogyneincognita (Kofoid et White). The system consists of a tomato plant, commercially available hydroponic culture medium, a 500-mL glass beaker, and an airstone connected to an air pump for aeration. The results show that a daily yield of more than 5000 J2s per plant can be obtained for at least for 20 days. Maximum yields of approximately 51,000–66,000 J2s per day were obtained between 11 and 21 days after initiation of the hydroponic culture. The infectivity of the J2s obtained was equivalent to that of J2s collected from egg masses.