Immunologic regulation of experimental cutaneous leishmaniasis. V. Characterization of effector and specific suppressor T cells.
Immunologic regulation of experimental cutaneous leishmaniasis. V. Characterization of effector and specific suppressor T cells.
复制标题
实验性皮肤利什曼病的免疫调节。
作者：
F. Liew;C. Hale;J. Howard
感染热带利什曼原虫前鞭毛体的抗性 CBA 小鼠会产生针对再次感染的伴随免疫力和恢复期免疫力。这可以通过脾和淋巴结 T 细胞过继转移，阈剂量为 1 至 2.5 x 10(7)。效应细胞具有Thy-1+、Lyt-1+2-表型。相同的免疫细胞群还将特异性 DTH 过继转移至热带乳杆菌，这受到主要组织相容性复合体的限制。另一方面，感染热带乳杆菌的高度易感性的 BALB/c 小鼠会发育出抗原特异性抑制性 T (Ts) 细胞（之前已显示可抑制 DTH 的诱导和表达），这些细胞在转移时能够逆转由先前对 BALB/c 小鼠进行亚致死照射诱导的损伤的愈合。只需 10(6) 个 T 细胞即可有效消除 550 rad 的强效预防作用。 Ts 细胞具有 Thy-1+、Lyt-1+2- 和 I-J- 表型。亚致死辐射和感染的 BALB/c 小鼠在感染后关键的前 40 天期间产生定量和同型相似的抗体反应，无论它们是否注射了 10(7) 个 Ts 细胞（未愈合与愈合）。因此，BALB/c 小鼠中 DTH 和治疗性免疫反应的损害不能归因于这些 Thy-1+、Lyt-1+2- 细胞形成抑制性抗体的辅助功能。
Resistant CBA mice infected with Leishmania tropica promastigotes develop concomitant and convalescent immunity against reinfection. This can be adoptively transferred by splenic and lymph node T cells with a threshold dosage of 1 to 2.5 x 10(7). The effector cells are of Thy-1+, Lyt-1+2- phenotype. The same immune cell population also adoptively transfers specific DTH to L. tropica, which is restricted by the major histocompatibility complex. On the other hand, highly susceptible BALB/c mice infected with L. tropica develop antigen-specific suppressor T (Ts) cells (previously shown to inhibit the induction and expression of DTH), which are capable, on transference, of reversing the healing of lesions induced by prior sublethal irradiation of BALB/c mice. As few as 10(6) of these T cells are effective in abrogating the potent prophylactic effect of 550 rad. The Ts cells are of Thy-1+, Lyt-1+2-, and I-J- phenotype. Sublethally irradiated and infected BALB/c mice produce antibody responses quantitatively and isotypically similar during the critical first 40 days after infection whether or not they are injected with 10(7) Ts cells (nonhealing vs healing). Thus, impairment of DTH and curative immune responses in BALB/c mice cannot be attributed to a helper function of these Thy-1+, Lyt-1+2- cells for the formation of suppressive antibody.