Sulforaphane alter the microbiota and mitigate colitis severity on mice ulcerative colitis induced by DSS
Sulforaphane alter the microbiota and mitigate colitis severity on mice ulcerative colitis induced by DSS
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萝卜硫素改变 DSS 诱导的小鼠溃疡性结肠炎的微生物群并减轻结肠炎的严重程度
DOI：
10.1186/s13568-020-01053-z
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发表时间：
2020-07-03
期刊：
影响因子：
3.7
通讯作者：
Zhang, Zhenyu
中科院分区：
文献类型：
--
作者：
Zhang, Yan;Tan, Luxuan;Zhang, Zhenyu
萝卜硫素是一种天然异硫氰酸酯，广泛存在于十字花科植物中。关于莱菔硫烷在溃疡性结肠炎中的抗炎作用的研究很少。本研究旨在探讨莱菔硫烷对UC小鼠肠道微生物群落的影响。以结肠长度、存活率、体重和疾病活动指数（DAI）评分来衡量小鼠结肠炎的严重程度。HE染色观察结肠组织形态学改变。采用16S rRNA基因扩增产物焦磷酸测序技术分析小鼠植物群的变化。采用定量PCR技术研究了不同浓度的植物群表达的变化。莱菔硫烷处理的小鼠比DSS诱导的小鼠具有更大的体重和更长的结肠长度。DSS组结肠组织充血水肿。同时，莱菔硫烷治疗可有效降低损伤评分和MPO活性。萝卜硫素逆转DSS诱导的肠道生态失调。萝卜硫素会把平衡转移到丁酸杆菌的炎症上。萝卜硫素的抗炎作用机制可能是与丁酸球菌等益生菌协同作用。综上所述，这些发现证明萝卜硫素可能是一个有用的内容，并作为一个潜在的治疗UC。
Sulforaphane (SFN) is a kind of natural isothiocyanate, which exists in cruciferous plants. Only few studies were about the anti-inflammatory effects of sulforaphane in ulcerative colitis. In this study, our purpose is to explore the effects of sulforaphane on the intestinal microbial community of UC mice. The severity of mice colitis were measured by colon length, survial rate, body weight and disease activity index (DAI) score. Histological and morphological evaluation of colon tissues were performed by HE. 16S rRNA gene amplicon pyrosequencing was used to analyza the changes of mouse flora. The variety of flora expression were explored using quantitative PCR. Sulforaphane treated mice had larger body weight and longer colon length than DSS-induced mice. The colon tissues of DSS group showed congestion and edema. Meanwhile, treatment with sulforaphane effectively reducted the damage scores and MPO activity. Sulforaphane reversed DSS-induced gut dysbiosis. Sulforaphane would shift the balance toButyricicoccuson inflammation. The possible anti-inflammatory mechanism of sulforaphane is to coordinate with the probiotics such asButyricicoccus. In summary, these findings proved that sulforaphane might be a useful content and serve as a potential therapy in the treatment of UC.