MUC1 in normal and impaired spermatogenesis
MUC1 in normal and impaired spermatogenesis
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DOI：
10.1093/molehr/7.6.505
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发表时间：
2001-06-01
影响因子：
4
通讯作者：
Bergmann, M
中科院分区：
文献类型：
--
作者：
Franke, FE;Kraus, S;Bergmann, M
MUC 1粘蛋白[也称为上皮唾液酸、上皮膜抗原（EMA）或多态性上皮粘蛋白（PEM）]是粘膜糖萼的一种组分，有助于抗粘附和保护细胞功能。MUC 1在男性和女性生殖道的多种上皮细胞类型中均有表达，但这是首次报道其在人类睾丸和生殖细胞谱系的非上皮细胞中表达。分析65个睾丸正常或受损的精子发生，我们确定MUC 1蛋白在成熟的生殖细胞免疫组化使用单克隆抗体HMFG 1，HMFG 2和SM 3结合不同的糖基化变体。MUC 1的表达通过逆转录-聚合酶链反应（RT-PCR）和Western印迹分析人睾丸组织提取物，以及紫外激光辅助细胞挑选后的选定生殖细胞的RT-PCR证实。MUC 1糖基化变异体在正常精子发生过程中选择性分布，而HMFG 1只标记粗线期精母细胞，HMFG 2只标记精细胞，未发现SM 3识别的低糖基化MUC 1粘蛋白，与其在正常精子发生过程中弱表达相反，HMFG 1糖基化变异体在所有成熟异常或停滞的精母细胞中显著积累，这些结果表明MUC 1粘蛋白在分化的生殖细胞中的可变糖基化，这在病理条件下是异常的。
The MUC1 mucin [also known as episialin, epithelial membrane antigen (EMA) or polymorphic epithelial mucin (PEM)] is a component of the mucosal glycocalyx, contributing to anti-adhesive and protective cell functions. MUC1 has been shown in a variety of epithelial cell types in the reproductive tracts of males and females, but this is the first report of its expression in human testis and non-epithelial cells of the germ cell lineage. Analysing 65 testes with normal or impaired spermatogenesis, we identified MUC1 protein in maturing germ cells by immunohistochemistry using the monoclonal antibodies HMFG1, HMFG2 and SM3 binding to different glycosylation variants. MUC1 expression was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis on tissue extracts of human testis, and RT-PCR of selected germ cells after UV laser-assisted cell picking. MUC1 glycosylation variants were selectively distributed during normal spermatogenesis, Whereas HMFG1 labelled certain groups of pachytene spermatocytes, HMFG2 labelled only spermatids, Low glycosylated forms of MUC1 mucin, recognized by SM3, were not found. In contrast to its weak expression during normal spermatogenesis, the HMFG1 glycosylation variant accumulated markedly in all spermatocytes showing abnormal or arrested maturation, These results suggest a variable glycosylation of MUC1 mucin in differentiating germ cells, which is aberrant in pathological conditions.