Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Helicobacter pylori strain 26695
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Helicobacter pylori strain 26695
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DOI：
10.1128/jb.186.4.949-955.2004
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发表时间：
2004-02-01
影响因子：
3.2
通讯作者：
Rhee, KH
中科院分区：
文献类型：
--
作者：
Baik, SC;Kim, KM;Rhee, KH
幽门螺杆菌引起胃十二指肠疾病，部分是由其外膜蛋白 (OMP) 介导的。为了鉴定幽门螺杆菌菌株 26695 的 OMP，我们进行了蛋白质组分析。通过使用固定化 pH 梯度条的二维电泳来解析肌氨酸不溶性外膜部分。大多数分子量为 10 至 100 kDa 的蛋白质斑点在凝胶上碱性 pI 区域（6.0 至 10.0）中可见。通过基质辅助激光解吸电离飞行时间质谱法分析 OMP 的蛋白质组。在处理的 80 个蛋白质点中，鉴定出 62 个点；它们代表了35个基因，其中包括16种OMP。此外，我们通过免疫印迹分析鉴定了 9 种免疫反应蛋白。本研究有助于对幽门螺杆菌菌株26695蛋白质组进行表征，并可能有助于进一步阐明幽门螺杆菌OMPs的生物学功能和幽门螺杆菌感染的发病机制。
Helicobacter pylori causes gastroduodenal disease, which is mediated in part by its outer membrane proteins (OMPs). To identify OMPs of H. pylori strain 26695, we performed a proteomic analysis. A sarcosine-insoluble outer membrane fraction was resolved by two-dimensional electrophoresis with immobilized pH gradient strips. Most of the protein spots, with molecular masses of 10 to 100 kDa, were visible on the gel in the alkaline pI regions (6.0 to 10.0). The proteome of the OMPs was analyzed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Of the 80 protein spots processed, 62 spots were identified; they represented 35 genes, including 16 kinds of OMP. Moreover, we identified 9 immunoreactive proteins by immunoblot analysis. This study contributes to the characterization of the H. pylori strain 26695 proteome and may help to further elucidate the biological function of H. pylori OMPs and the pathogenesis of H. pylori infection.