The histidine permease gene (HIP1) of Saccharomyces cerevisiae.
The histidine permease gene (HIP1) of Saccharomyces cerevisiae.
复制标题
酿酒酵母的组氨酸通透酶基因 (HIP1)。
DOI：
10.1016/0378-1119(85)90219-7
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发表时间：
1985
期刊：
影响因子：
3.5
通讯作者：
Fink,GR
中科院分区：
文献类型：
--
作者：
Tanaka,J;Fink,GR
本研究对酿酒酵母（Saccharomyces gulae）的组氨酸特异性通透酶基因（HIP 1）进行了定位、克隆和序列测定。HIP 1基因定位于染色体VII的右臂，约1000 bp。在ADE 3基因远端11 cM处。该基因被分离为一个8.6 kb的BamHIW-Sau 3A片段的组氨酸特异性通透酶缺陷受体酵母细胞的互补。我们对该片段的一个2.4kb的亚片段进行了测序，并鉴定了一个1596 bp的开放阅读框架（ORF）。通过测序证实该1596-bp的ORF为HIP 1编码序列。对HIP 1基因氨基酸序列的分析显示了几个疏水片段，但没有发现明显的N端信号肽。我们在体外构建了一个缺失的HIP 1基因，并用该缺失替换了该基因的野生型拷贝。hipl缺失突变体在补充30 mM组氨酸（50倍于HIP 1细胞生长所需量）时可以生长。这种缺失突变体的回复突变体能够在组氨酸的正常水平上生长，这是由非连锁基因的突变引起的。这两个观察结果表明，有额外的，低亲和力的组氨酸摄取途径。
The histidine-specific permease gene (HIP1) ofSaccharomyces cerevisiaehas been mapped, cloned, and sequenced. TheHIP1gene maps to the right arm of chromosome VII, approx. 11 cM distal to theADE3gene. The gene was isolated as an 8.6-kbBamHIW-Sau3A fragment by complementation of the histidine-specific permease deficiency in recipient yeast cells. We sequenced a 2.4-kb subfragment of thisBamHI-Sau3A fragment containing theHIP1gene and identified a 1596-bp open reading frame (ORF). We confirmed the assignment of the 1596-bp ORF as theHIP1coding sequence by sequencing ahiplnonsense mutation. Analysis of the amino acid (aa) sequence of theHIP1gene reveals several hydrophobic stretches, but shows no obvious N-terminal signal peptide. We have constructed a deletion of theHIP1gene in vitro and replaced the wild-type copy of the gene with this deletion. Thehipldeletion mutant can grow when it is supplemented with 30 mM histidine, 50 times the amount required for the growth ofHIP1cells. Revertants of this deletion mutant able to grow on a normal level of histidine arise by mutation in unlinked genes. Both these observations suggest that there are additional, low-affinity pathways for histidine uptake.