Senescence marker protein 30 (SMP30)/regucalcin (RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish
Senescence marker protein 30 (SMP30)/regucalcin (RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish
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DOI：
10.1016/j.bbrc.2011.09.067
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发表时间：
2011-10-22
影响因子：
3.1
通讯作者：
Sakaida, Isao
中科院分区：
文献类型：
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作者：
Fujisawa, Koichi;Terai, Shuji;Sakaida, Isao
衰老标记蛋白30（SMP 30）/regucalcin（RGN）与衰老、肝细胞增殖和肿瘤发生有关。然而，非哺乳动物SMP 30/RGN的表达和功能知之甚少。我们发现，斑马鱼SMP 30/RGN mRNA的表达随着年龄的增长，部分肝切除和硫代乙酰胺诱导的急性肝损伤而下降。SMP 30/RGN表达在斑马鱼肝细胞系中也大大降低。此外，我们通过给予二乙基亚硝胺在成年斑马鱼中诱导肝肿瘤。与周围区域相比，在病灶、肝细胞癌、胆管细胞癌和混合瘤中观察到表达降低。因此，我们显示了SMP 30/RGN在肝增殖和肿瘤发生中的重要性。(C)2011 Elsevier Inc. All rights reserved.
Senescence marker protein 30 (SMP30)/regucalcin (RGN) is known to be related to aging, hepatocyte proliferation and tumorigenesis. However, expression and function of non-mammalian SMP30/RGN is poorly understood. We found that zebrafish SMP30/RGN mRNA expression decreases with aging, partial hepatectomy and thioacetamide-induced acute liver injury. SMP30/RGN expression was also greatly decreased in a zebrafish liver cell line. In addition, we induced liver tumors in adult zebrafish by administering diethylnitrosamine. Decreased expression was observed in foci, hepatocellular carcinomas, cholangiocellular carcinomas and mixed tumors as compared to the surrounding area. We thus showed the importance of SMP30/RGN in liver proliferation and tumorigenesis. (C) 2011 Elsevier Inc. All rights reserved.