Characterization of Ehrlichia species from Ixodes ovatus ticks at the foot of Mt. Fuji, Japan
Characterization of Ehrlichia species from Ixodes ovatus ticks at the foot of Mt. Fuji, Japan
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DOI：
10.1111/j.1348-0421.2004.tb03599.x
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Ohashi, N
中科院分区：
文献类型：
--
作者：
Inayoshi, M;Naitou, H;Ohashi, N
共采集成虫390只(卵形硬蜱288只，全沟硬蜱102只)。对日本静冈县富士及其附近的两个城市进行了埃立克体感染的检测，方法是用实验室小白鼠从整个硬蜱组织中分离。从接种卵形艾美耳球虫组织的小鼠的脾中检测到埃立克希菌DNA，但没有检测到全硫代艾美耳球虫。伊立克次体在硬蜱中的流行率估计约为3%。16S rDNA分析表明，获得的8个埃利希菌分离物(定名为静冈分离物)的序列与最近在日本分离到的两个埃立克体种变异株的序列非常相似，但不完全相同，其次是美国的查菲埃利希氏菌。对单核细胞埃立克体病病原OMP-1多基因家族的部分分析表明，静冈分离株与其他埃立克体菌有所不同。静冈分离株在免疫能力强的实验室小鼠中导致死亡，表明它们对小鼠具有高致病性。资料表明，静冈分离株很可能是日本埃立克体属的一个新变种。由于这些类似人类埃立克体病的生物的存在，日本将需要进一步的定性和监测。
A total of 390 adult ticks (288 Ixodes ovatus and 102 I. persulcatus) collected at the foot of Mt. Fuji and two near cities in Shizuoka prefecture, Japan, were examined for Ehrlichia infection by isolation with laboratory mice from whole tick tissues. Ehrlichial DNAs were detected from the spleens of mice inoculated with tissues from I. ovatus, but not I. persulcatus. The prevalence of ehrlichiae in the ticks was estimated to be ca. 3%. The 16S rDNA analysis revealed that the sequences of 8 ehrlichial isolates (termed "Shizuoka" isolates) obtained were identical, and they were very similar, but not identical, to those of two Ehrlichia species strain variants recently isolated in Japan, followed by Ehrlichia chaffeensis in the US. Analysis of parts of the omp-1 multigene family specific for monocytic ehrlichiosis agents showed that the Shizuoka isolates were distinct from other ehrlichial organisms. The Shizuoka isolates caused death in immunocompetent laboratory mice, suggesting that they are highly pathogenic in mice. The data show that the Shizuoka isolates are likely to be a new strain variant of Ehrlichia species in Japan. Further characterization and surveillance will be required in Japan due to the presence of these human ehrlichiosis agent-like organisms.