MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis
MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis
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DOI：
10.1105/tpc.107.057422
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发表时间：
2008-02-01
期刊：
影响因子：
11.6
通讯作者：
Favery, Bruno
中科院分区：
文献类型：
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作者：
Caillaud, Marie-Cecile;Lecomte, Philippe;Favery, Bruno
专性寄生线虫对植物的侵染为研究植物细胞骨架功能提供了一个有趣的模型。根结线虫已经进化出操纵宿主功能的能力，通过将根细胞再分化为多核和肥大的喂养细胞。这些巨细胞是在没有细胞分裂的情况下由反复的核分裂产生的。详细的功能分析表明，拟南芥微管相关蛋白65-3 （MAP65-3）对巨细胞的形成至关重要，细胞分裂在巨细胞中开始但未完成。在发育中的巨细胞中，MAP65-3与一种新的细胞板——巨细胞迷你细胞板——有关，这种细胞板可以分离子细胞核。在缺少功能性MAP65-3的情况下，巨细胞发育但不能完全分化，最终被破坏。巨细胞的这些缺陷损害了线虫幼虫的成熟。因此，MAP65-3在根结线虫感染过程中对巨细胞发育至关重要。在dyc283 T-DNA MAP65-3突变体中，MAP65-3的亚细胞定位和微管组织分析表明，MAP65-3在所有植物器官有丝分裂早期和晚期组织有丝分裂微管阵列中发挥了关键作用。在这里，我们提出了MAP65-3在巨细胞个体发生中的作用模型。
The infection of plants by obligate parasitic nematodes constitutes an interesting model for investigating plant cytoskeleton functions. Root knot nematodes have evolved the ability to manipulate host functions to their own advantage by redifferentiating root cells into multinucleate and hypertrophied feeding cells. These giant cells result from repeated rounds of karyokinesis without cell division. Detailed functional analyses demonstrated that Arabidopsis thaliana Microtubule-Associated Protein65-3 (MAP65-3) was essential for giant cell ontogenesis and that cytokinesis was initiated but not completed in giant cells. In developing giant cells, MAP65-3 was associated with a novel kind of cell plate - the giant cell mini cell plate - that separates daughter nuclei. In the absence of functional MAP65-3, giant cells developed but failed to fully differentiate and were eventually destroyed. These defects in giant cells impaired the maturation of nematode larvae. Thus, MAP65-3 is essential for giant cell development during root knot nematode infection. Subcellular localization of MAP65-3 and analysis of microtubule organization in the dyc283 T-DNA map65-3 mutant demonstrated that MAP65-3 played a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs. Here, we propose a model for the role of MAP65-3 in giant cell ontogenesis.