Characterization of Enterocoliticin, a Phage Tail-Like Bacteriocin, and Its Effect on Pathogenic Yersinia enterocolitica Strains
Characterization of Enterocoliticin, a Phage Tail-Like Bacteriocin, and Its Effect on Pathogenic Yersinia enterocolitica Strains
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小肠结肠菌素（一种噬菌体尾样细菌素）的特性及其对致病性小肠结肠炎耶尔森菌菌株的影响
DOI：
10.1128/aem.67.12.5634-5642.2001
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发表时间：
2001
影响因子：
4.4
通讯作者：
B. Appel
中科院分区：
文献类型：
--
作者：
E. Strauch;H. Kaspar;C. Schaudinn;P. Dersch;K. Madela;C. Gewinner;S. Hertwig;J. Wecke;B. Appel
摘要 小肠结肠炎耶尔森氏菌 29930（生物群 1A；血清群 O：7,8）产生一种细菌素，称为小肠结肠炎素，对属于血清群 O:3、O:5,27 和 O:9 的小肠结肠炎耶尔森菌肠道致病菌株具有抑制活性。纯化小肠大肠菌素，小肠大肠菌素制剂的电子显微照片显示存在噬菌体尾状颗粒。这些颗粒不含核酸，在与敏感细菌接触时表现出收缩。在易感菌株的对数期培养物中添加小肠结肠素可导致 CFU 快速剂量依赖性降低。对敏感细菌培养物热输出的量热测量表明，添加小肠结肠素后细胞代谢活性立即完全丧失。此外，确定了 K+ 离子流入培养基的剂量依赖性流出，表明小肠结肠菌素具有通道形成活性。
ABSTRACT Yersinia enterocolitica 29930 (biogroup 1A; serogroup O:7,8) produces a bacteriocin, designated enterocoliticin, that shows inhibitory activity against enteropathogenic strains of Y. enterocolitica belonging to serogroups O:3, O:5,27 and O:9. Enterocoliticin was purified, and electron micrographs of enterocoliticin preparations revealed the presence of phage tail-like particles. The particles did not contain nucleic acids and showed contraction upon contact with susceptible bacteria. Enterocoliticin addition to logarithmic-phase cultures of susceptible bacterial strains led to a rapid dose-dependent reduction in CFU. Calorimetric measurements of the heat output of cultures of sensitive bacteria showed a complete loss of cellular metabolic activity immediately upon addition of enterocoliticin. Furthermore, a dose-dependent efflux of K+ ions into the medium was determined, indicating that enterocoliticin has channel-forming activity.