Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote glioma progression by sponging miR-126-5p
Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote glioma progression by sponging miR-126-5p
复制标题
长非编码 RNA HOTAIR 充当竞争性内源 RNA，通过海绵 miR-126-5p 促进神经胶质瘤进展
DOI：
10.1002/jcp.26432
复制
发表时间：
2018-09-01
影响因子：
5.6
通讯作者：
Shi, Yan
中科院分区：
文献类型：
--
作者：
Liu, Liang;Cui, Sitong;Shi, Yan
LncRNA HOX转录物反义基因间RNA（HOTAIR）已被证明在肿瘤发生中起重要作用。然而，其在胶质瘤中的确切分子机制尚未完全阐明。在本研究中，我们发现HOTAIR在胶质瘤组织中异常上调，并与miR-126- 5 p表达呈负相关。接下来，我们确定HOTAIR通过海绵状miR-126- 5 p促进胶质瘤进展。随后，使用生物信息学软件和荧光素酶报告基因测定法证实了谷氨酰胺酶（GLS）是miR-126- 5 p的直接靶点。此外，HOTAIR可以通过作为miR-126- 5 p的竞争性内源RNA（ceRNA）来调节GLS表达。综上所述，我们的研究阐明了HOTAIR/miR-126/GLS通路参与胶质瘤进展，并可能作为胶质瘤治疗的靶点。
LncRNA HOX transcript antisense intergenic RNA (HOTAIR) has been shown to play prominent roles in tumorigenesis. However, its precise molecular mechanism in glioma has not been completely elucidated. In this study, we found that HOTAIR was aberrantly up-regulated in glioma tissues and was negatively correlated with miR-126-5p expression. Next, we determined that HOTAIR promote glioma progression by sponging miR-126-5p. Subsequently, glutaminase (GLS) was confirmed to be a direct target of miR-126-5p using bioinformatics software and a luciferase reporter assay. Moreover, HOTAIR could modulate GLS expression by functioning as a competing endogenous RNA (ceRNA) for miR-126-5p. Taken together, our study clarified that the HOTAIR/miR-126/GLS pathway is involved in glioma progression and may potentially serve as a target for glioma therapy.