Facile Fabrication of Thin-Bottom Round-Well Plates Using the Deformation of PDMS Molds and Their Application for Single-Cell PCR
Facile Fabrication of Thin-Bottom Round-Well Plates Using the Deformation of PDMS Molds and Their Application for Single-Cell PCR
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DOI：
10.3390/mi11080748
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发表时间：
2020-08-01
期刊：
影响因子：
3.4
通讯作者：
Heike, Yuji
中科院分区：
文献类型：
--
作者：
Yamahira, Shinya;Heike, Yuji
最近，由树脂制成的微型器件在从基础生命科学研究到医学应用的一系列领域中有力地支持了细胞分析。许多微型器件是通过将树脂模制到由刚性材料精确制成的模具中来制造的。然而，由于模具中的尺寸误差也被精确地打印到产品上，因此产品的精度被限制为小于刚性模具的精度。因此，我们假设，如果尺寸误差可以自我校正的弹性模具，微器件可以轻松地制造精度超过模具。在本文中，我们报告了一种新的加工策略，其中由聚甲基硅氧烷（PDMS）制成的弹性模具变形以补偿产品的尺寸误差。通过使用具有384个PDMS凸起的模具热压成型聚碳酸酯板，该PDMS凸起具有标准偏差为+/-15.6 μ m的高度的大尺寸误差，容易地制造底部厚度为13.3 +/-2.3 μ m的384-圆孔板（n= 384）。最后，单细胞观察和聚合酶链反应（PCR）证明了弹性PDMS模具制成的产品的应用。因此，这种加工方法是一种很有前途的策略，方便，低成本，和更高的精度微加工。
Recently, microdevices made of resins have been strongly supporting cell analysis in a range of fields, from fundamental life science research to medical applications. Many microdevices are fabricated by molding resin to a mold made precisely from rigid materials. However, because dimensional errors in the mold are also accurately printed to the products, the accuracy of the product is limited to less than the accuracy of the rigid mold. Therefore, we hypothesized that if dimensional errors could be self-corrected by elastic molds, microdevices could be facilely fabricated with precision beyond that of molds. In this paper, we report a novel processing strategy in which an elastic mold made of polymethylsiloxane (PDMS) deforms to compensate for the dimensional error on the products. By heat-press molding a polycarbonate plate using a mold that has 384 PDMS convexes with a large dimensional error of height of +/- 15.6 mu m in standard deviation, a 384-round-well plate with a bottom thickness 13.3 +/- 2.3 mu m (n= 384) was easily fabricated. Finally, single-cell observation and polymerase chain reactions (PCRs) demonstrated the application of the products made by elastic PDMS molds. Therefore, this processing method is a promising strategy for facile, low-cost, and higher precision microfabrication.