MONOCLONAL-ANTIBODY REVEALS RADIAL GLIA IN ADULT AVIAN BRAIN
MONOCLONAL-ANTIBODY REVEALS RADIAL GLIA IN ADULT AVIAN BRAIN
复制标题
DOI：
10.1002/cne.902640203
复制
发表时间：
1987-10-08
影响因子：
2.5
通讯作者：
NOTTEBOHM, F
中科院分区：
文献类型：
--
作者：
ALVAREZBUYLLA, A;BUSKIRK, DR;NOTTEBOHM, F
一种针对成年金丝雀脑制备的抗体，40 E-C，染色室区细胞，这些细胞将长而不分支的突起送入前脑实质。我们将这些细胞鉴定为放射状神经胶质。同样的抗体也会染色一部分脑星形胶质细胞，并与非脑物质如间充质、支持细胞和肌肉的Z线反应。红细胞和某些内皮细胞的反应较弱。40 E-C还与发育中的大鼠脑的放射状神经胶质反应，但在成年啮齿动物脑中未显示任何此类神经胶质。蛋白质印迹分析表明，该抗体识别波形蛋白，这是所有40种E-C阳性细胞类型共有的分子。我们认为，放射状胶质细胞在成年鸟类前脑的存在和它们在哺乳动物中的明显缺失与成年期的神经发生有关，这发生在鸟类中，而在哺乳动物中很少或根本没有。此外，成年鸟类放射状神经胶质细胞的存在也可能与成年鸟类和哺乳动物大脑生理学上的其他差异有关。
An antibody prepared against adult canary brain, 40E-C, stains ventricular zone cells that send long, unbranched processes into the forebrain parenchyma. We identify these cells as radial glia. The same antibody also stains a subset of brain astroglia and reacts with nonbrain material such as mesenchyme, Sertoli cells, and the Z-line of muscle. A weaker reaction is given by erythrocytes and some endothelial cells. 40E-C also reacts with the radial glia of the developing rat brain but fails to show any such glia in adult rodent brain. Western blot analysis shows that this antibody recognizes vimentin, a molecule shared by all 40E-C positive cell types. We believe that the presence of radial glia in the adult avian forebrain and their apparent absence in mammals is related to neurogenesis in adulthood, which occurs in birds and much less or not at all in mammals. In addition, the presence of radial glia in adult birds may also relate to other, still hypothetical, differences in the physiology of adult avian and mammalian brains.