Sophorolipid biosurfactants: Possible uses as antibacterial and antibiofilm agent
Sophorolipid biosurfactants: Possible uses as antibacterial and antibiofilm agent
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DOI：
10.1016/j.nbt.2015.02.009
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发表时间：
2015-12-25
影响因子：
5.4
通讯作者：
Martin, Peter J.
中科院分区：
文献类型：
--
作者：
De Rienzo, Mayri A. Diaz;Banat, Ibrahim M.;Martin, Peter J.
生物表面活性剂是微生物来源的两亲性表面活性分子，它们聚集在界面上，降低界面张力，并在溶液中形成聚集的微细胞结构。据报道，一些生物表面活性剂具有抗菌特性，即防止粘连和破坏生物膜形成的能力。我们研究了不同浓度的苦参脂类的抗菌性能和对生物膜的破坏。5%苦豆脂对革兰氏阴性铜绿假单胞菌ATCC 17699和革兰氏阳性枯草杆菌BBK006的生长均有抑制作用。扫描电子显微镜观察到，在静态和流动条件下，苦参脂(5%v/v)也能破坏枯草杆菌BBK006和金黄色葡萄球菌ATCC 9144单一和混合培养形成的生物膜。结果表明，苦参脂类化合物可能通过抑制生长和/或破坏生物膜而作为其他抗菌剂的辅助剂在生物医学应用中应用。
Biosurfactants are amphipathic, surface-active molecules of microbial origin which accumulate at interfaces reducing interfacial tension and leading to the formation of aggregated micellular structures in solution. Some biosurfactants have been reported to have antimicrobial properties, the ability to prevent adhesion and to disrupt biofilm formation. We investigated antimicrobial properties and biofilm disruption using sophorolipids at different concentrations. Growth of Gram negative Cupriavidus necator ATCC 17699 and Gram positive Bacillus subtilis BBK006 were inhibited by sophorolipids at concentrations of 5% v/v with a bactericidal effect. Sophorolipids ( 5% v/v) were also able to disrupt biofilms formed by single and mixed cultures of B. subtilis BBK006 and Staphylococcus aureus ATCC 9144 under static and flow conditions, as was observed by scanning electron microscopy. The results indicated that sophorolipids may be promising compounds for use in biomedical application as adjuvants to other antimicrobial against some pathogens through inhibition of growth and/or biofilm disruption.