Amino acid distribution in soil humic acids fractionated by tandem size exclusion chromatography polyacrylamide gel electrophoresis
Amino acid distribution in soil humic acids fractionated by tandem size exclusion chromatography polyacrylamide gel electrophoresis
复制标题
串联尺寸排阻色谱聚丙烯酰胺凝胶电泳分离土壤腐植酸中的氨基酸分布
DOI：
10.1016/s0160-4120(98)00036-1
复制
发表时间：
1998
影响因子：
11.8
通讯作者：
O. Trubetskoj
中科院分区：
文献类型：
--
作者：
O. Trubetskaya;O. Reznikova;G. Afanas'eva;L. F. Markova;T. Muranova;O. Trubetskoj
采用体积排阻色谱-聚丙烯酰胺凝胶电泳法对北极土、灰化土、灰林土、黑钙土和红壤中的胡敏酸及其组分的氨基酸组成和含量进行了研究。级分具有精确定义的分子大小（MS）和电泳迁移率（EM）。虽然HA和馏分具有非常相似的氨基酸组成，但它们的氨基酸含量差异很大。高MS组分中氨基酸含量最高（11.5-21.7%）。氨基酸含量基本上随着洗脱体积和HA组分EM的增加而降低（至2.1-5.5%），与HA来源无关。将HA分离成具有确定的分子和电泳实体的不太复杂的级分，其氨基酸含量不同，对于研究HA的物理化学结构及其在土壤中的发生具有重要价值。
The objective of this work was to study humic acids (HAs), and their fractions obtained by coupling size exclusion chromatography-polyacrylamide gel electrophoresis, from arctic soil, podzolic soil, grey forest soil, chernozem and red soil, in respect to their amino acids composition and content. The fractions had exactly defined molecular size (MS) and electrophoretic mobilities (EM). While HAs and fractions had remarkably similar amino acids compositions, they differed greatly in amino acids contents. The highest quantity of amino acids was found in high MS fractions (11.5–21.7%). Amino acids content essentially decreased (to 2.1–5.5%) with the increasing of elution volumes and EM of HAs fractions, independently of HA sources. Separation of HA into less complex fractions with defined molecular and electrophoretic entities, which differ by their content of amino acids, could be of great value for studies on physical-chemical structures of HA and its genesis in soil.