A screening lateral flow immunochromatographic assay for on-site detection of okadaic acid in shellfish products
A screening lateral flow immunochromatographic assay for on-site detection of okadaic acid in shellfish products
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贝类产品中冈田酸现场筛查侧流免疫色谱法
DOI：
10.1016/j.ab.2011.12.039
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发表时间：
2012-03-15
影响因子：
2.9
通讯作者：
Liu, Zeng-Shan
中科院分区：
文献类型：
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作者：
Lu, Shi-Ying;Lin, Chao;Liu, Zeng-Shan
建立了一种基于胶体金-单抗(McAb)结合物的横向流动免疫层析(LFIC)试纸，用于现场快速检测贝类中冈田酸(OA)。它应用了一种竞争形式，使用样品中存在的固定化毒素结合物和游离毒素。以20 nm胶体金为检测试剂，制备了抗OA单抗。样品中的毒素与固定化毒素竞争结合到与McAb偶联的金上。胶体金/单抗/毒素流动络合物在试验线上不被牛血清白蛋白(BSA)捕获，而在对照线上被山羊抗鼠免疫球蛋白G(IgG)捕获。测试品系的色密度与毒素浓度在10~50 ng·ml~(-1)范围内呈正相关。样品的定性检出限为150微克公斤(-1)，接近欧盟(EU)的限量(160微克公斤(-1))。因此，这些试纸能够直接、定性地评价贝类的食用安全性。他们不需要设备，因为可以获得视觉效果，他们在10分钟内筛选了大量样本。高效液相色谱-串联质谱仪进一步证实了这一结果。作为一种食品安全筛查工具，该试纸方便、实用，可用于现场快速检测贝类产品中OA的存在。(C)2012 Elsevier Inc.保留所有权利。
A lateral flow immunochromatographic (LFIC) test strip based on a colloidal gold-monoclonal antibody (McAb) conjugate was developed for on-site rapid detection of okadaic acid (OA) in shellfish. It applies a competitive format using an immobilized toxin conjugate and free toxin present in samples. The McAb against OA was conjugated with 20-nm colloidal gold as detector reagent. The toxin in the sample competed with the immobilized toxin to bind to the gold conjugated with McAb. The colloidal gold/McAb/toxin mobile complex was not captured by OA-bovine serum albumin (BSA) on the test line, but it was captured by goat anti-mouse immunoglobulin G (IgG) on the control line. The color density of the test line correlated with the concentration of toxin in the range of 10-50 ng ml(-1). The qualitative detection limit of 150 mu g kg(-1) sample was close to the European Union (EU) regulatory limit (160 mu g kg(-1)). Therefore, these strips were able to directly and qualitatively estimate the consuming safety of shellfish. They require no equipment because of available visual results, and they screened numerous samples within 10 min. The results were further confirmed by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). As a food safety screening tool, the test strips are convenient and useful to rapidly on-site test the presence of OA in shellfish products. (C) 2012 Elsevier Inc. All rights reserved.