Human hair follicle bulge cells are biochemically distinct and possess an epithelial stem cell phenotype
Human hair follicle bulge cells are biochemically distinct and possess an epithelial stem cell phenotype
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DOI：
10.1038/sj.jidsp.5640233
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发表时间：
1999-12-01
影响因子：
--
通讯作者：
Cotsarelis, G
中科院分区：
文献类型：
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作者：
Lyle, S;Christofidou-Solomidou, M;Cotsarelis, G
干细胞对于自我更新组织的稳态是至关重要的，并且它们的操纵可能具有广泛的应用，包括基因治疗、伤口修复和组织移植。虽然啮齿类动物的毛囊干细胞已经定位于毛囊隆起，但人类毛囊干细胞的位置不太清楚，并且由于缺乏隆起区域的细胞标记物而阻碍了它们的表征。我们证明，C8/144 B单克隆抗体，最初提出的对CD 8肽序列，免疫染色的人毛囊隆起。我们发现，这种抗体识别角质形成细胞中的细胞角蛋白15（K15），K15阳性隆突细胞具有干细胞表型，其特征在于其缓慢的周期性质，在新毛囊生长开始时增殖，和高水平的β（1）整合素表达。这些结果将人毛囊干细胞定位于隆突，并表明K15优先在上皮干细胞中表达。
Stem cells are vital for the homeostasis of self-renewing tissues and their manipulation may have wide ranging applications, including gene therapy, wound repair, and tissue transplantation. Although rodent hair follicle stem cells have been localized to the follicle bulge, the location of human hair follicle stem cells is less clear, and their characterization has been hampered by a lack of cellular markers for the bulge area. We demonstrate that the C8/144B monoclonal antibody, originally raised against a CD8 peptide sequence, immunostains the human hair follicle bulge. We show that this antibody recognizes cytokeratin 15 (K15) in keratinocytes, and that K15-positive bulge cells possess a stem cell phenotype characterized by their slowly cycling nature, proliferation at the onset of new hair follicle growth, and high level of beta(1) integrin expression. These results localize human hair follicle stem cells to the bulge and suggest that K15 is preferentially expressed in epithelial stem cells.