INDUCTION OF CELL FATE IN THE DROSOPHILA RETINA - THE BRIDE OF SEVENLESS PROTEIN IS PREDICTED TO CONTAIN A LARGE EXTRACELLULAR DOMAIN AND 7 TRANSMEMBRANE SEGMENTS
INDUCTION OF CELL FATE IN THE DROSOPHILA RETINA - THE BRIDE OF SEVENLESS PROTEIN IS PREDICTED TO CONTAIN A LARGE EXTRACELLULAR DOMAIN AND 7 TRANSMEMBRANE SEGMENTS
复制标题
DOI：
10.1101/gad.4.11.1835
复制
发表时间：
1990-11-01
影响因子：
10.5
通讯作者：
ZIPURSKY, SL
中科院分区：
文献类型：
--
作者：
HART, AC;KRAMER, H;ZIPURSKY, SL
先前的遗传镶嵌研究确定，果蝇的新娘sevenless（老板）基因的表达是感光神经元R8感光神经元R7的发展所必需的。这导致了boss编码或调节R7特异性诱导线索的建议。我们已经根据突变等位基因中的小缺失鉴定了boss基因，并对cDNA和相应的基因组区域进行了测序。一个P元件和三个X射线诱导的boss等位基因在基因中显示不同的缺失，大小从2到23 bp不等，每一个都引起移码，导致翻译的过早终止。boss基因编码896个氨基酸的蛋白质，其具有推定的氨基末端信号序列、498个氨基酸的大胞外区和7个潜在的跨膜结构域，随后是115个氨基酸的羧基末端胞质尾。推定的膜定位的老板蛋白是一致的模型，其中老板和sevenless蛋白之间的直接相互作用指定R7细胞的命运。另一个模型中，老板蛋白作为一个受体的功能提出了基于其相似性的G蛋白连接的家庭的膜受体。
Previous genetic mosaic studies established that expression of the Drosophila bride of sevenless (boss) gene is required in photoreceptor neurons R8 for the development of photoreceptor neuron R7. This led to the proposal that boss encodes or regulates an R7-specific inductive cue. We have identified the boss gene based on small deletions in mutant alleles and sequenced both cDNAs and corresponding genomic regions. One P element and three X-ray-induced boss alleles show different deletions in the gene ranging in size from 2 to 23 bp, each causing frameshifts leading to premature termination of translation. the boss gene encodes a protein of 896 amino acids with a putative amino-terminal signal sequence, a large extracellular region of 498 amino acids, and seven potential transmembrane domains followed by a carboxy-terminal cytoplasmic tail of 115 amino acid. The putative membrane localization of the boss protein is consistent with a model in which direct interaction between the boss and sevenless proteins specifies R7 cell fate. Another model in which the boss protein functions as a receptor is proposed based on its similarity to the G protein-linked family of membrane receptors.