Polymorphism in the ELOVL6 gene is associated with a major QTL effect on fatty acid composition in pigs.
Polymorphism in the ELOVL6 gene is associated with a major QTL effect on fatty acid composition in pigs.
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DOI：
10.1371/journal.pone.0053687
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ballester M
中科院分区：
文献类型：
--
作者：
Corominas J;Ramayo-Caldas Y;Puig-Oliveras A;Pérez-Montarelo D;Noguera JL;Folch JM;Ballester M
脂肪酸延长酶6（FIGHVL 6）是唯一与从头脂肪生成相关的延长酶，其通过控制哺乳动物中的脂肪酸平衡来催化延长周期中的限速步骤。它位于猪8号染色体（SSC 8）的一个区域，其中影响棕榈酸和棕榈油酸组成的QTL先前被检测到，使用伊比利亚×长白猪互交。这项工作的主要目标是精细定位QTL，并评估作为一个位置候选基因影响的棕榈酸和棕榈油酸脂肪酸在猪的百分比的ERFVL 6基因。基于单倍型的方法和单标记分析的组合使我们能够确定QTL的主要相关区间，其中确定并选择了ESTVL 6基因作为位置候选基因。一个多态性在启动子区的p53 VL 6，p53 VL 6：c.- 533 C>T与肌肉和背膘中棕榈酸和棕榈油酸的百分比高度相关。当动物通过BLV 6分类时，在背膘中观察到BLV 6基因表达的显著差异：533 C>T基因型。因此，携带与HPVL 6基因表达降低相关的等位基因的动物呈现出C16：0和C16：1（n-7）脂肪酸含量的增加以及肌肉和背膘中伸长活性比的降低。此外，一项SNP全基因组关联研究显示，在背膘中，HPVL 6相对表达水平对HPVL 6基因所在的SSC 8区域的影响最强。最后，GWAS还在肝脏和肌肉中鉴定了与HPVL 6基因表达相关的不同潜在基因组区域，表明HPVL 6基因的差异组织调节。我们的研究结果表明，HPLVL 6作为一个潜在的致病基因的QTL分析，随后，控制猪的脂肪酸组成的整体平衡。
The ELOVL fatty acid elongase 6 (ELOVL6), the only elongase related to de novo lipogenesis, catalyzes the rate-limiting step in the elongation cycle by controlling the fatty acid balance in mammals. It is located on pig chromosome 8 (SSC8) in a region where a QTL affecting palmitic, and palmitoleic acid composition was previously detected, using an Iberian x Landrace intercross. The main goal of this work was to fine-map the QTL and to evaluate the ELOVL6 gene as a positional candidate gene affecting the percentages of palmitic and palmitoleic fatty acids in pigs. The combination of a haplotype-based approach and single-marker analysis allowed us to identify the main, associated interval for the QTL, in which the ELOVL6 gene was identified and selected as a positional candidate gene. A polymorphism in the promoter region of ELOVL6, ELOVL6:c.-533C>T, was highly associated with the percentage of palmitic and palmitoleic acids in muscle and backfat. Significant differences in ELOVL6 gene expression were observed in backfat when animals were classified by the ELOVL6:c.-533C>T genotype. Accordingly, animals carrying the allele associated with a decrease in ELOVL6 gene expression presented an increase in C16:0 and C16:1(n-7) fatty acid content and a decrease of elongation activity ratios in muscle and backfat. Furthermore, a SNP genome-wide association study with ELOVL6 relative expression levels in backfat showed the strongest effect on the SSC8 region in which the ELOVL6 gene is located. Finally, different potential genomic regions associated with ELOVL6 gene expression were also identified by GWAS in liver and muscle, suggesting a differential tissue regulation of the ELOVL6 gene. Our results suggest ELOVL6 as a potential causal gene for the QTL analyzed and, subsequently, for controlling the overall balance of fatty acid composition in pigs.
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影响因子：
64.5
作者：
Cao H;Gerhold K;Mayers JR;Wiest MM;Watkins SM;Hotamisligil GS
通讯作者：
Hotamisligil GS
影响因子：
3.5
作者：
Erkens T;Van Poucke M;Vandesompele J;Goossens K;Van Zeveren A;Peelman LJ
通讯作者：
Peelman LJ
影响因子：
2.4
作者：
Estelle, J.;Fernandez, A. I.;Folch, J. M.
通讯作者：
Folch, J. M.
DOI：
10.1016/j.bbrc.2008.01.075
发表时间：
2008-04-04
影响因子：
3.1
作者：
Kumadaki, Shin;Matsuzaka, Takashi;Shimano, Hitoshi
通讯作者：
Shimano, Hitoshi
影响因子：
7.1
作者：
Aarsland, A;Chinkes, D;Wolfe, RR
通讯作者：
Wolfe, RR