Nitric oxide synthase-inhibition hypertension is associated with altered endothelial cyclooxygenase function.
Nitric oxide synthase-inhibition hypertension is associated with altered endothelial cyclooxygenase function.
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一氧化氮合酶抑制性高血压与内皮环氧合酶功能改变有关。
DOI：
10.1152/ajpheart.00628.2004
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Kanagy,NancyL
中科院分区：
文献类型：
--
作者：
Bratz,IanN;Kanagy,NancyL
We reported previously that endothelium-intact superior mesenteric arteries (SMA) fromNω-nitro-l-arginine (l-NNA)-treated hypertensive rats (LHR) contract more to norepinephrine (NE) than SMA from control rats. Others have shown that nitric oxide (NO) synthase (NOS) inhibition increases cyclooxygenase (COX) function and expression. We hypothesized that augmented vascular sensitivity to NE in LHR arteries is caused by decreased NOS-induced dilation and increased COX product-induced constriction. We observed that the EC50for NE is lower in LHR SMA compared with control SMA (control −6.37 ± 0.04, LHR −7.89 ± 0.09 log mol/l;P< 0.05). Endothelium removal lowered the EC50(control −7.95 ± 0.11, LHR −8.44 ± 0.13 log mol/l;P< 0.05) and increased maximum tension in control (control 1,036 ± 38 vs. 893 ± 21 mg;P< 0.05) but not LHR (928 ± 30 vs. 1,066 ± 31 mg) SMA. Thus augmented NE sensitivity in LHR SMA depends largely on decreased endothelial dilation. NOS inhibition (l-NNA, 10−4mol/l) increased maximum tension and EC50in control arteries but not in LHR arteries. In contrast, COX inhibition decreased maximum tension in control arteries, suggesting that COX products augment contraction. Indomethacin did not affect NE-induced contraction inl-NNA-treated or denuded arteries. In control SMA loaded with the fluorescent NO indicator 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate, indomethacin increased andl-NNA decreased NO release. Therefore, COX products appear to inhibit NO production to augment NE-induced contraction. With chronic NOS inhibition, this modulating influence is greatly diminished. Thus, in NOS-inhibition hypertension, decreased activity of both COX and NOS pathways profoundly disrupts endothelial modulation of contraction.
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DOI：
--
发表时间：
1999-12
期刊：
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
影响因子：
--
作者：
V. Brovkovych;L. Dobrucki;S. Brovkovych;I. Dobrucki;C. A. D. do Nascimento;A. Burewicz;T. Malinski
通讯作者：
V. Brovkovych;L. Dobrucki;S. Brovkovych;I. Dobrucki;C. A. D. do Nascimento;A. Burewicz;T. Malinski
影响因子：
8.3
作者：
KATO, T;IWAMA, Y;SATAKE, T
通讯作者：
SATAKE, T
影响因子：
3.2
作者：
KISHI, K;INOUE, T
通讯作者：
INOUE, T
影响因子：
6
作者：
Millgård, J;Lind, L
通讯作者：
Lind, L
DOI：
10.1172/jci14172
发表时间：
2003-04
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
U. Landmesser;S. Dikalov;S. Price;Louise A. McCann;T. Fukai;S. Holland;W. Mitch;D. Harrison
通讯作者：
U. Landmesser;S. Dikalov;S. Price;Louise A. McCann;T. Fukai;S. Holland;W. Mitch;D. Harrison