INVIVO EXCISION OF PYRIMIDINE DIMERS IS MEDIATED BY A DNA N-GLYCOSYLASE IN MICROCOCCUS-LUTEUS BUT NOT IN HUMAN-FIBROBLASTS
INVIVO EXCISION OF PYRIMIDINE DIMERS IS MEDIATED BY A DNA N-GLYCOSYLASE IN MICROCOCCUS-LUTEUS BUT NOT IN HUMAN-FIBROBLASTS
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DOI：
10.1111/j.1751-1097.1982.tb04381.x
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
LINN, S
中科院分区：
文献类型：
--
作者：
LABELLE, M;LINN, S
先前已经表明，藤黄微球菌具有嘧啶二聚体特异性核酸内切酶，其在体外既作为核酸内切酶又作为DNA糖基化酶。为了确定这些组合活性是否在体内起作用，我们分离并检查了UV辐照M的切除产物。黄体。此外，我们还设计了一种方法来分离和检查UV照射的人成纤维细胞的切除产物，以确定内切核酸酶/糖基化酶活性是否在人成纤维细胞中UV诱导的嘧啶二聚体的切除中起作用。我们发现，在体内，核酸内切酶/糖基化酶机制被广泛用于修复嘧啶二聚体由M。黄体，但人成纤维细胞似乎不使用这种机制。
It has been previously shown thatMicrococcus luteuspossesses a pyrimidine dimer‐specific endonuclease whichin vitro, functions as both an endonuclease and DNA‐glycosylase. To determine if these combined activities functionin vivo, we have isolated and examined the excision products of UV‐irradiatedM. luteus. In addition, we have devised a procedure to isolate and examine the excision products from UV‐irradiated human fibroblasts to determine if an endonuclease/glycosylase activity functions in the excision of UV‐induced pyrimidine dimers in human fibroblasts. We find that,in vivo, an endonuclease/glycosylase mechanism is utilized extensively in the repair of pyrimidine dimers byM. luteus, but that human fibroblasts do not appear to use this mechanism.