OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice.
OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice.
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OsIRO3 在缺铁反应和调节水稻铁稳态中发挥重要作用
DOI：
10.3390/plants9091095
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发表时间：
2020-08-25
期刊：
影响因子：
--
通讯作者：
Zheng L
中科院分区：
文献类型：
--
作者：
Wang W;Ye J;Ma Y;Wang T;Shou H;Zheng L
铁稳态是植物生长发育所必需的，它受到一组转录因子的严格调控。铁相关转录因子3（OsIRO 3）是水稻缺铁反应的负调控因子。然而，OsIRO 3调节Fe稳态的分子机制尚不清楚。在这里，我们报告说，OsIRO 3是必不可少的响应缺铁和维持铁的稳态在水稻。OsIRO 3在根、叶和基节中表达，在营养生长期叶片中表达量较高。OsIRO 3基因的敲除导致对缺铁的敏感性，幼叶严重坏死，根发育不良。在缺铁条件下，iro 3突变体在地上部中积累了更高水平的铁，这与上调OsNAS 3的表达相关，这导致烟酰胺（NA）在根中的积累增加。进一步分析表明OsIRO 3可以直接与OsNAS 3启动子中的E-box结合。此外，典型的铁相关基因的表达显着上调iro 3突变体在铁充足的条件下。因此，我们得出结论，OsIRO 3在响应铁缺乏和调节NA水平的直接，负调控OsNAS 3的表达发挥了关键作用。
Iron (Fe) homeostasis is essential for plant growth and development, and it is strictly regulated by a group of transcriptional factors. Iron-related transcription factor 3 (OsIRO3) was previously identified as a negative regulator for Fe deficiency response in rice. However, the molecular mechanisms by which OsIRO3 regulate Fe homeostasis is unclear. Here, we report that OsIRO3 is essential for responding to Fe deficiency and maintaining Fe homeostasis in rice. OsIRO3 is expressed in the roots, leaves, and base nodes, with a higher level in leaf blades at the vegetative growth stage. Knockout of OsIRO3 resulted in a hypersensitivity to Fe deficiency, with severe necrosis on young leaves and defective root development. The iro3 mutants accumulated higher levels of Fe in the shoot under Fe-deficient conditions, associated with upregulating the expression of OsNAS3, which lead to increased accumulation of nicotianamine (NA) in the roots. Further analysis indicated that OsIRO3 can directly bind to the E-box in the promoter of OsNAS3. Moreover, the expression of typical Fe-related genes was significantly up-regulated in iro3 mutants under Fe-sufficient conditions. Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the OsNAS3 expression.
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DOI：
10.1074/jbc.m110.180026
发表时间：
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期刊：
The Journal of biological chemistry
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DOI：
10.1073/pnas.1916892116
发表时间：
2019-12-10
影响因子：
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