Estimation of Evolutionary Rates of Mitochondrial DNA in Two Japanese Wood Mouse Species Based on Calibrations with Quaternary Environmental Changes
Estimation of Evolutionary Rates of Mitochondrial DNA in Two Japanese Wood Mouse Species Based on Calibrations with Quaternary Environmental Changes
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DOI：
10.2108/zs160169
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发表时间：
2017-06-01
影响因子：
0.9
通讯作者：
Suzuki, Hitoshi
中科院分区：
文献类型：
--
作者：
Hanazaki, Kaori;Tomozawa, Morihiko;Suzuki, Hitoshi
线粒体DNA的进化速率的可靠估计可能使我们能够建立现实的进化场景覆盖广泛的时间尺度的基础上系统发育推断。在本研究中，我们试图获得估计的进化率在啮齿类动物使用校准对历史地理事件。我们首先假设，陆桥状结构，间歇性地出现在冰川最大的10万年的间隔形状的发散模式的细胞色素B（CytB）序列（1140 bp）的较大的日本林鼠美姬鼠。通过比较被深深的海峡分隔开的偏远岛屿的序列，我们可以估计线粒体DNA的进化速率（替换/位点/百万年）为0.027 ~ 0.036，并假定在14万年、25万年、35万年和44万年前进行了校准。其次，我们解决了快速扩张事件推断从分析的Cytb序列的小日本木小鼠A。阿让提斯我们在数据集中检测到五个扩展信号，并根据扩展参数tau值建立了三个类别：3.9，5.6-5.7和7.8-8.1。考虑到气候在15，000年、53，000年和115，000年前的快速冷却之后变得更加温暖，我们计算出的进化速率分别为0.114、0.047和0.031。这个关于木鼠在15，000年至440，000年前时间尺度上进化速率的初步概念应该在其他啮齿类动物中得到完善和测试，包括小鼠和大鼠。
Reliable estimates of evolutionary rates of mitochondrial DNA might allow us to build realistic evolutionary scenarios covering broad time scales based on phylogenetic inferences. In the present study, we sought to obtain estimates of evolutionary rates in murine rodents using calibrations against historical biogeographic events. We first assumed that land-bridge-like structures that appeared intermittently at glacial maxima with 100,000-year intervals shaped the divergence patterns of cytochrome b (Cytb) sequences (1140 bp) of the larger Japanese wood mouse Apodemus speciosus. The comparison of sequences from peripheral remote islands that are separated from one another by deep straits allowed us to estimate mitochondrial DNA evolutionary rates (substitutions/site/million years) to be 0.027 to 0.036, with presumed calibrations from 140,000, 250,000, 350,000, and 440,000 years ago. Second, we addressed rapid expansion events inferred from analyses of the Cytb sequences of the lesser Japanese wood mouse A. argenteus. We detected five expansion signals in the dataset and established three categories based on the expansion parameter tau values: 3.9, 5.6-5.7, and 7.8-8.1. Considering that the climate became warmer 15,000, 53,000, and 115,000 years ago after preceding periods of rapid cooling, we calculated evolutionary rates to be 0.114, 0.047, and 0.031, respectively. This preliminary concept of the evolutionary rates on a time scale from 15,000 to 440,000 years ago for the wood mouse should be refined and tested in other species of murine rodents, including mice and rats.