Neuropathology in Depth: The Role of Confocal Microscopy
Neuropathology in Depth: The Role of Confocal Microscopy
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深度神经病理学：共焦显微镜的作用
DOI：
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发表时间：
1992
影响因子：
3.2
通讯作者：
J. Murray
中科院分区：
文献类型：
--
作者：
J. Murray
This review surveys the rapidly increasing applications of confocal microscopy in neuropathology and related areas of experimental neurology. The ability of this new instrument to “optically section” thick samples has opened up to microscopic examination a wide range of previously difficult specimens. The basic operating principle, trading field of view for axial resolution, is illustrated with a simple schematic comparison of conventional and confocal microscopes. Images can be collected in several different modes, utilizing fluorescence, reflectance, or transmitted light. The majority of the applications in neuropathology to date have focused on munocytochemical labeling with peroxidase or fluorescent markers. New methods of specimen preparation designed explicitly for the confocal microscope are just beginning to appear. The trend toward new techniques that fully exploit the superior performance of confocal microscopes is expected to accelerate, which will quickly establish the confocal microscope as a routine laboratory tool for the neuropathologist.
