Total internal reflection fluorescence microscopy in single molecule nanobioscience
Total internal reflection fluorescence microscopy in single molecule nanobioscience
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DOI：
10.1007/b102211
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Ueda, M
中科院分区：
文献类型：
--
作者：
Wazawa, T;Ueda, M
全内反射荧光显微镜（TIRFM）的最新发展使得在体外和活细胞中在单分子水平上直接监测生物分子的行为成为可能。这项技术开创了生物科学的新纪元，即所谓的单分子纳米生物科学。本文综述了TIRFM的理论基础，介绍了TIRFM用于单分子成像的仪器，并讨论了TIRFM在单分子纳米生物科学中的一些新应用。
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at the single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience.