Preparation of cell aggregates incorporating gelatin hydrogel microspheres of sugar-responsive water-solubilization
Preparation of cell aggregates incorporating gelatin hydrogel microspheres of sugar-responsive water-solubilization
复制标题
糖响应性水溶明胶水凝胶微球细胞聚集体的制备
DOI：
10.1002/term.307
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发表时间：
2020
影响因子：
3.3
通讯作者：
Tabata Yasuhiko
中科院分区：
文献类型：
--
作者：
Inoo Kanako;Yamamoto Masaya;Tabata Yasuhiko
由于关节软骨的无血管和无神经性质，其自我再生能力有限。加载的外植体和细胞培养物表明，软骨细胞代谢可以通过生理负荷调节。然而，明确的范围内的机械刺激，对应于有利的代谢反应与细胞外基质（ECM）的合成是难以捉摸的。缺乏这方面知识的非系统性方案会产生不一致的结果。本研究的目的是确定增加ECM合成的物理刺激的内在范围，同时抑制一氧化氮（NO）的生产在软骨细胞琼脂糖结构，通过数值重新评估的实验进行Tsuang等。（2008年）。在ABAQUS中用多孔弹性有限元模型模拟了12种加载模式。固体基质上的压力，冯米塞斯应力，最大主应力和孔隙压力被选为内在的力学刺激。在结构水平上，用MATLAB计算了循环载荷稳态下它们的发展速率和幅度。在2300 Pa压力和40 Pa/s压力速率下观察到糖胺聚糖和胶原蛋白的同时增加。在0-1500 Pa和0-40 Pa/s之间，NO的产生相对于对照始终是阳性的，而ECM的合成在相同范围内是阴性的。压力速率与NO生成量呈线性相关（R= 0.77）。在这项研究中确定的应力状态是通用的，并可用于开发预测算法的任意形状，大小和琼脂糖浓度的琼脂糖-软骨细胞结构中的基质生产。它们也可能有助于提高无血管组织工程的负载方案的功效。版权所有© 2010约翰威利父子有限公司。
The self-regeneration capacity of articular cartilage is limited, due to its avascular and aneural nature. Loaded explants and cell cultures demonstrated that chondrocyte metabolism can be regulated via physiologic loading. However, the explicit ranges of mechanical stimuli that correspond to favourable metabolic response associated with extracellular matrix (ECM) synthesis are elusive. Unsystematic protocols lacking this knowledge produce inconsistent results. This study aims to determine the intrinsic ranges of physical stimuli that increase ECM synthesis and simultaneously inhibit nitric oxide (NO) production in chondrocyte–agarose constructs, by numerically reevaluating the experiments performed by Tsuang et al.(2008). Twelve loading patterns were simulated with poro-elastic finite element models in ABAQUS. Pressure on solid matrix, von Mises stress, maximum principle stress and pore pressure were selected as intrinsic mechanical stimuli. Their development rates and magnitudes at the steady state of cyclic loading were calculated with MATLAB at the construct level. Concurrent increase in glycosaminoglycan and collagen was observed at 2300 Pa pressure and 40 Pa/s pressure rate. Between 0–1500 Pa and 0–40 Pa/s, NO production was consistently positive with respect to controls, whereas ECM synthesis was negative in the same range. A linear correlation was found between pressure rate and NO production (R= 0.77). Stress states identified in this study are generic and could be used to develop predictive algorithms for matrix production in agarose–chondrocyte constructs of arbitrary shape, size and agarose concentration. They could also be helpful to increase the efficacy of loading protocols for avascular tissue engineering. Copyright 2010 John Wiley & Sons, Ltd.