Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 x Wangshuibai population. II: Type I resistance
Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 x Wangshuibai population. II: Type I resistance
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DOI：
10.1007/s00122-005-0156-3
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发表时间：
2006-02-01
影响因子：
5.4
通讯作者：
Ma, ZQ
中科院分区：
文献类型：
--
作者：
Lin, F;Xue, SL;Ma, ZQ
小麦赤霉病是小麦和大麦上的一种严重病害，严重影响小麦的产量和品质。为了鉴定抗病基因，在4种不同环境下对"南大2419" × "望水白" RIL群体及其亲本的感病穗率进行了测定。利用构建的2,960cM分子标记框架图，利用Mapmaker/QTL进行区间定位，共检测到10个与Ⅰ型抗性相关的染色体区域，其中位于4B染色体上Xwmc 349与Xgwm 149相似区间的QTL，Xwmc 96在5A染色体上与Xgwm304相似，Xgwm408在5B染色体上与Xbarc 140相似，至少在三种环境中发现了Xwmc 96和Xgwm408的抗性等位基因。Qfhi.nau-4B和Qfhi.nau-5A的效应较大，分别解释了17.5%和27.0%的表型变异。为了检测上位性QTL，利用全基因组扫描检测了两个位点的互作。5个位点对的互作对Ⅰ型抗病性有显著影响，LOD值在3.8 - 6.5之间，其中4个位点对在亲本阶段就表现出抗病性。具有最显著效果的是Xcfd 42（类似于Xgwm 469（6D））/Xwmc 390 - 2（类似于Xbd 04（2A））对。未检测到PIS的QTL × E互作。结果表明，开花时间对该群体的PIS没有显著影响。研究表明，望水白在抗赤霉病育种中具有良好的应用价值，与抗赤霉病QTL相关的分子标记可用于分子标记辅助选择和抗赤霉病QTL的分离。
Fusarium head blight (FHB) is a serious disease in wheat and barley affecting both yield and quality. To identify genes for resistance to infection, the RIL population derived from 'Nanda2419' x 'Wangshuibai' and the parents were evaluated for percentage of infected spikes (PIS) in four different environments. Using a 2,960 cM marker framework map constructed for this population, ten chromosome regions were detected for their association with type I resistance through interval mapping with Mapmaker/QTL, among which QTLs mapped in the intervals of Xwmc349 similar to Xgwm149 on chromosome 4B, of Xwmc96 similar to Xgwm304 on chromosome 5A and of Xgwm408 similar to Xbarc140 on chromosome 5B were revealed in at least three environments and have Wangshuibai as the source of resistance alleles. Qfhi.nau-4B and Qfhi.nau-5A had larger effects and explained up to 17.5 and 27.0% of the phenotypic variance, respectively. To detect epistasis QTLs, two-locus interactions were examined by whole genome scan. Interactions of five locus pairs were found to have significant effects on type I resistance with the LOD score ranging 3.8-6.5 and four of them conferred resistance in parental phase. The one with the most significant effect was Xcfd42 similar to Xgwm469 (6D)/Xwmc390-2 similar to Xbd04 (2A) pair. No QTL x E interaction was detected for PIS. It was found that flowering time did not have significant effects on PIS in this population. Our studies indicated that Wangshuibai is useful for breeding for both type I and type II scab resistance and the markers associated with the QTLs could be used in marker-assisted selection and isolation of scab-resistance QTLs.