Adaptive response in mice exposed to 900 MHZ radiofrequency fields: Bleomycin-induced DNA and oxidative damage/repair
Adaptive response in mice exposed to 900 MHZ radiofrequency fields: Bleomycin-induced DNA and oxidative damage/repair
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DOI：
10.3109/09553002.2014.980465
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发表时间：
2015-01
影响因子：
2.6
通讯作者：
C. Zong;Yongxin Ji;Qina He;Shunxing Zhu;Fenju Qin;J. Tong;Yi Cao
中科院分区：
文献类型：
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作者：
C. Zong;Yongxin Ji;Qina He;Shunxing Zhu;Fenju Qin;J. Tong;Yi Cao
摘要目的：目的：研究小鼠暴露于射频场（RF）后注射拟放射性药物博莱霉素（BLM）是否会产生适应性反应，并为这种反应提供机制依据。材料与方法：将成年小鼠暴露于900 MHz、功率密度为120 μW/cm 2的射频中，每天4 h，连续7 d。在最后一次暴露后立即处死一些小鼠，而另一些小鼠在4 h后注射BLM。在每只动物中：（i）在血液白细胞中测定原发性DNA损伤和BLM诱导的损伤及其修复动力学;（ii）从血浆、肝和肺组织中的丙二醛（MDA）水平测定氧化损伤，并从超氧化物歧化酶（SOD）水平评估抗氧化状态。结果：有没有迹象表明，增加DNA和氧化损伤的小鼠暴露于RF单独与BLM单独处理的相反。RF+ BLM组小鼠DNA损伤明显减轻，且在30、60、90、120和150 min修复后DNA损伤仍存在;（B）血浆和肝脏MDA含量降低，肺组织SOD含量升高。结论：总体数据表明，RF暴露能够诱导适应性反应，并通过激活某些细胞过程减轻BLM诱导的DNA和氧化损伤。
Abstract Purpose: To determine whether mice exposed to radiofrequency fields (RF) and then injected with a radiomimetic drug, bleomycin (BLM), exhibit adaptive response and provide some mechanistic evidence for such response. Materials and methods: Adult mice were exposed to 900 MHz RF at 120 μW/cm2 power density for 4 hours/day for 7 days. Immediately after the last exposure, some mice were sacrificed while the others were injected with BLM 4 h later. In each animal: (i) The primary DNA damage and BLM-induced damage as well as its repair kinetics were determined in blood leukocytes; and (ii) the oxidative damage was determined from malondialdehyde (MDA) levels and the antioxidant status was assessed from superoxide dismutase (SOD) levels in plasma, liver and lung tissues. Results: There were no indications for increased DNA and oxidative damages in mice exposed to RF alone in contrast to those treated with BLM alone. Mice exposed to RF+ BLM showed significantly: (a) reduced BLM-induced DNA damage and that remained after each 30, 60, 90, 120 and 150 min repair time, and (b) decreased levels of MDA in plasma and liver, and increased SOD level in the lung. Conclusions: The overall data suggested that RF exposure was capable of inducing adaptive response and mitigated BLM- induced DNA and oxidative damages by activating certain cellular processes.