Expression of CD44 in rat hepatic progenitor cells
Expression of CD44 in rat hepatic progenitor cells
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DOI：
10.1016/j.jhep.2006.01.029
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发表时间：
2006-07-01
影响因子：
25.7
通讯作者：
Mitaka, Toshihiro
中科院分区：
文献类型：
--
作者：
Kon, Junko;Ooe, Hidekazu;Mitaka, Toshihiro
背景/目的：小肝细胞（SHs）是肝祖细胞，但SHs和成熟肝细胞（MHs）之间的表型差异从未被证实。方法：利用DNA芯片检测SHs和MHs的基因表达谱，以明确SHs和MHs之间的差异。鉴定了SHs中特异性表达的基因，并对其进行了RT-PCR分析。用培养的SHs和d -半乳糖胺（GaIN）损伤的大鼠肝脏对CD44标准型（CD44s）和变异型6 （CD44v6）进行免疫细胞化学检测。从经gain处理的肝脏中，通过分选获得CD44s（+）细胞，并进行RT-PCR分析。结果：经DNA芯片和RT-PCR分析，发现SHs中CD44s和CD44v6表达受限。在培养中，cd44在第3天出现，并随着SHs的增殖而增加。与CD44s相比，CD44v6表达延迟。在给药后，CD44（+）肝细胞在第3天和第4天出现在门静脉周围，然后减少。分类后的CD44s+细胞可形成集落并具有肝脏标记物。结论：CD44是SHs的特异性标志物。CD44 mRNA和蛋白的表达仅限于SHs，在体外和体内均在SHs开始增殖时上调。(c) 2006欧洲肝脏研究协会。Elsevier B.V.版权所有。
Background/Aims: Small hepatocytes (SHs) are hepatic progenitor cells, but the phenotypical difference between SHs and mature hepatocytes (MHs) has never been demonstrated.Methods: The profile of gene expression was examined to clarify the difference between SHs and MHs by using a DNA microarray. Genes that were specifically expressed in SHs were identified and RT-PCR analysis of them was performed. Immunocytochemistry for CD44 standard form (CD44s) and variant form 6 (CD44v6) was performed using cultured SHs and the D-galactosamine (GaIN)-injured rat liver. From the GaIN-treated liver, CD44s(+) cells were obtained by sorting and RT-PCR analysis was performed.Results: Analysis using the DNA microarray and RT-PCR of them revealed restricted expression of CD44s and CD44v6 in SHs. In culture, CD44s appeared at day 3 and increased with the proliferation of SHs. CD44v6 expression was delayed compared to that of CD44s. With GaIN-administration, CD44(+) hepatocytes appeared around periportal areas at days 3 and 4 and then decreased. Sorted CD44s+ cells could form colonies and possessed hepatic markers.Conclusions: CD44 is a specific marker of SHs. The expression of CD44 mRNA and protein is restricted to SHs, and is up-regulated at the time when SHs start to proliferate both in vitro and in vivo. (c) 2006 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.