Study on effect of diluent osmotic pressure on yeast cell strength and cell size using a novel micromanipulation technique
Study on effect of diluent osmotic pressure on yeast cell strength and cell size using a novel micromanipulation technique
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使用新型显微操作技术研究稀释剂渗透压对酵母细胞强度和细胞大小的影响
DOI：
10.1023/a:1008892116065
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发表时间：
1998
影响因子：
4.1
通讯作者：
T. Srinorakutara
中科院分区：
文献类型：
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作者：
T. Srinorakutara
一种新的显微操作技术以前被用来测量单个动物细胞的机械特性，现在已经应用于酵母细胞。在这项研究中，该技术被用于测量酵母细胞强度和细胞大小跨越2l批发酵。另外，也可以使用库尔特计数器测定细胞大小，同时用导电流体（Isoton II）稀释细胞测量值。对于细胞强度，发现稀释剂的渗透压确实影响细胞强度。然而，我们也发现，稀释剂的渗透压对细胞大小没有显著的影响，无论使用库尔特计数器或显微操作进行测量。显微操作已被证明是一种强大的技术，用于测量酵母细胞的机械特性，它将非常有用的研究他们的行为在细胞破坏设备，如高压均质机。
A new micromanipulation technique which has previously been used to measure the mechanical properties of single animal cells has now been applied to yeast cells. In this study this technique was used to measure yeast cell strength and cell size across a 2l batch fermentation. Alternatively the cell size could also be determined using a Coulter counter while cell measurement was diluted with a conducting fluid (Isoton II). For the cell strength, it was found that the osmotic pressure of diluents did affect cell strength. However, it was also found that there was no significant effect of osmotic pressure of diluents on cell size whether a Coulter counter or micromanipulation was used for measurement. Micromanipulation has been shown to be a powerful technique for measuring the mechanical properties of yeast cells and it will be very useful for studying their behaviour in cell disruption equipment, e.g. high-pressure homogenizers.