THE IS10 TRANSPOSASE MESSENGER-RNA IS DESTABILIZED DURING ANTISENSE RNA CONTROL
THE IS10 TRANSPOSASE MESSENGER-RNA IS DESTABILIZED DURING ANTISENSE RNA CONTROL
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DOI：
10.1002/j.1460-2075.1990.tb08234.x
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发表时间：
1990-04-01
期刊：
影响因子：
11.4
通讯作者：
SIMONS, RW
中科院分区：
文献类型：
--
作者：
CASE, CC;SIMONS, EL;SIMONS, RW
RNA稳定性是基因表达的重要组成部分，已经提出反义RNA来改变靶RNA的稳定性。我们在这里表明，IS 10转座酶mRNA，RNA-IN，是不稳定的IS 10反义RNA，RNA-OUT控制期间。去稳定化需要RNA-OUT/RNA-IN配对和核糖核酸酶III切割。不依赖于这种切割，RNA-OUT通过破坏其二级结构而变得不稳定。配对对RNA-IN转录或稳定性没有其他明显影响。然而，RNA-IN去稳定化不是体内反义控制所必需的。在随附的论文中[Ma，C.和Simons，R.W. 05 The Dog of the Dog（1990）9，1267-1274]我们显示配对阻断核糖体与RNA-IN的结合。如果不是在这一层面上进行控制，不稳定将发挥更重要的作用。
RNA stability is an important component of gene expression, and antisense RNAs have been proposed to alter target RNA stability. We show here that the IS10 transposase mRNA, RNA-IN, is rendered unstable during control by the IS10 antisense RNA, RNA-OUT. Destabilization requires RNA-OUT/RNA-IN pairing and ribonuclease III cleavage. Independent of such cleavage, RNA-OUT is rendered unstable through disruption of its secondary structure. Pairing has no other obvious effects on RNA-IN transcription or stability. Nevertheless, RNA-IN destabilization is not required for antisense control in vivo. In the accompanying paper [Ma,C. and Simons,R.W. (1990) EMBO J., 9, 1267-1274] we show that pairing blocks ribosome binding to RNA-IN. Were it not for control at this level, destabilization would play a more important role.