Preparation and evaluation of proline-based chiral columns
Preparation and evaluation of proline-based chiral columns
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DOI：
10.1021/ac050050s
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发表时间：
2005-05-15
影响因子：
7.4
通讯作者：
Li, TY
中科院分区：
文献类型：
--
作者：
Huang, JM;Zhang, P;Li, TY
制备了由容易获得的脯氨酸肽制成的手性固定相，并对其进行了一般手性分离的评估。通过适当的结构元件，这些色谱柱表现出广泛的手性选择性。在测试的 53 种分析物中，四脯氨酸色谱柱分离了 31 种。这些分析物实现的分离效果与 Whelk O2 色谱柱实现的分离效果相当，但仍低于 Daicel AD-H 和 OD-H 色谱柱实现的分离效果。脯氨酸单元的数量被证明对于手性识别很重要，因为用单个脯氨酸单元制成的对照柱基本上是无效的。
Chiral stationary phases made of readily available proline peptides were prepared and evaluated for general chiral separation., With the proper structural elements, these columns demonstrated broad chiral selectivity. Among the 53 analytes tested, a tetraproline column resolved 31. The separations achieved for these analytes are comparable to those achieved on Whelk O2 column, while still inferior to those achieved on Daicel AD-H and OD-H columns. Number of proline units proves important for chiral recognition, because a control column made with a single proline unit is largely ineffective.