Effect of controlled zinc release on bone mineral density from injectable Zn-containing β-tricalcium phosphate suspension in zinc-deficient diseased rats
Effect of controlled zinc release on bone mineral density from injectable Zn-containing β-tricalcium phosphate suspension in zinc-deficient diseased rats
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DOI：
10.1002/jbm.a.30040
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发表时间：
2004-06-01
影响因子：
4.9
通讯作者：
Higuchi, WI
中科院分区：
文献类型：
--
作者：
Otsuka, M;Ohshita, Y;Higuchi, WI
本研究的目的是使用卵巢切除大鼠模型评估含锌（Zn）的β-磷酸三钙（Zn-TCP）纠正骨质疏松症中注意到的骨矿物质缺乏的有效性。四个实验组（D 0、D10、D20和N10）各使用四只大鼠。D 0、D10和D20组大鼠切除卵巢，喂饲维生素D、钙和锌缺乏饲料，诱导缺锌性糖尿病9周。N10组为正常大鼠，正常饮食喂养9周。D 0组注射纯β-TCP悬浮液，D10和D20组分别注射含有10 mol %（6.17 wt % Zn）和20 mol %（12.05 wt % Zn）Zn-TCP的悬浮液10 mg，健康组N10注射10 mol %。Zn-TCP悬浮液。每周一次在所有大鼠的左大腿肌内注射，并喂食维生素D和锌缺乏的饮食9周。测定各组大鼠血浆钙、锌水平、碱性磷酸酶活性及腰椎、股骨骨密度。所有大鼠的血浆锌水平在1.1和2.8 μ g/mL之间。计算0 - 63天之间Ca、Zn和ALP（Ca-AUC、Zn-AUC和ALP-AUC）水平的曲线下面积。结果表明：（1）Zn-AUC的大小顺序为N10 = D20 > D10 > D 0;（2）D 0、D10组的Ca-AUC显著低于N10组;（3）D10和D20组的ALP-AUC显著高于N10组，D 0组介于两者之间。D10和D20组的体重随时间显著增加，D 0组略有增加，N10组在整个实验期间保持不变。D10和D20组腰椎骨密度（约100 mg/cm 2）显著高于D 0组，但低于N10组。随着悬浮液中锌含量的增加，左股骨的BMD增加幅度大于右股骨。这项研究的结果表明，对BMD的局部影响比对全身的影响更明显。(C)2004 Wiley Periodicals，Inc.
The purpose of this study was to evaluate the efficacy of zinc (Zn)-containing beta-tricalcium phosphate (Zn-TCP) in correcting the bone mineral deficiency noted in osteoporosis using ovariectomized rat model. Four rats were used for each of the four experimental groups: D0, D10, D20, and N10. The rats in D0, D10, and D20 groups were ovariectomized, and fed a vitamin D-, Ca-, and Zn-deficient diet, and induced Zn-deficient osteoporoses for 9 weeks. In contrast, the N10 group was the normal rats fed normal healthy diet for 9 weeks. D0 group was injected with pure beta-TCP suspension, D10 and D20 groups were injected with suspensions containing 10 mg of 10 mol % (6.17 wt % Zn) and 20 mol % (12.05 wt % Zn) Zn-TCP, respectively, and the healthy group, N10 were injected with 10 mol %. Zn-TCP suspensions. Injections were administered intramuscularly in the left thigh once a week in all rats, and fed a vitamin D- and Zn-deficient diet for 9 weeks. The plasma calcium (Ca) and Zn levels, plasma alkaline phosphatase activity (ALP) and bone mineral density (BMD) of the lumbar vertebra and femora were measured. The plasma Zn levels in all the rats were between 1.1 and 2.8 mug/mL. The areas under the curves for the Ca, Zn, and ALP (Ca-AUC, Zn-AUC, and ALP-AUC) levels between 0 and 63 days were calculated. Results for the AUCs were as follows: (1) the Zn-AUCs were in the order of N10 = D20 > D10 > D0; (2) the Ca-AUCs for D0, D10 groups were significantly lower than that for the N10 group; (3) the ALP-AUCs for the D10 and D20 groups were significantly higher than that for the N10 group, and that of the D0 group was in between those. The body weight of D10 and D20 groups significantly increased with time, that of the D0 group increased slightly, and that of the N10 group remained unchanged for the entire experimental period. The BMD of the lumbar vertebrae of the D10 and D20 groups (about 100 mg/cm(2)) was significantly higher than that of the D0 group but lower than that of the N10 group. The BMD of the left femur increased more than that of the right femur with the increase in the amount of Zn in the suspension. The results of this study suggest that the local effect on BMD was more pronounced than the effect on the whole body. (C) 2004 Wiley Periodicals, Inc.