Complete mitochondrial genome of the medicinal fungus Ophiocordyceps sinensis.
Complete mitochondrial genome of the medicinal fungus Ophiocordyceps sinensis.
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药用真菌冬虫夏草完整线粒体基因组
DOI：
10.1038/srep13892
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发表时间：
2015-09-15
影响因子：
4.6
通讯作者：
Yao YJ
中科院分区：
文献类型：
--
作者：
Li Y;Hu XD;Yang RH;Hsiang T;Wang K;Liang DQ;Liang F;Cao DM;Zhou F;Wen G;Yao YJ
作为冬虫夏草1229菌株基因组测序计划的一部分，一个完整的线粒体(Mt)基因组被组装成一个157,510个碱基的环状dsDNA，这是已报道的真菌中最大的dsDNA之一。发现了包括大小rRNA亚基的保守基因，27个tRNA和15个蛋白质编码基因。此外，在基因间隔区和内含子区域还发现了58个非保守的开放阅读框(NcORF)。使用RNA-Seq的转录分析验证了大多数保守基因和ncORF的表达。保守基因内含52个内含子(组I和组II)，占基因组的68.5%。在I组内含子中鉴定出32个具有LAGLIDADG(21)和GIY-YIG(11)模式的同源内切酶(HES)。在第II组内含子中发现的ncORF主要编码逆转录酶。中华稻瘟病菌的mt基因组与其他亚克里亚目真菌一样，基因内容和序列都是保守的，但基因组大小因较长的基因间隔区和大量的内含子而变大。基因间隔区和内含子区域由丰富的重复序列组成，通常与可移动元件有关。编码RTS和HES的内含子ncORFs可能是中华绒螯蟹mt基因组扩增的原因之一。
As part of a genome sequencing project for Ophiocordyceps sinensis, strain 1229, a complete mitochondrial (mt) genome was assembled as a single circular dsDNA of 157,510 bp, one of the largest reported for fungi. Conserved genes including the large and small rRNA subunits, 27 tRNA and 15 protein-coding genes, were identified. In addition, 58 non-conserved open reading frames (ncORFs) in the intergenic and intronic regions were also identified. Transcription analyses using RNA-Seq validated the expression of most conserved genes and ncORFs. Fifty-two introns (groups I and II) were found within conserved genes, accounting for 68.5% of the genome. Thirty-two homing endonucleases (HEs) with motif patterns LAGLIDADG (21) and GIY-YIG (11) were identified in group I introns. The ncORFs found in group II introns mostly encoded reverse transcriptases (RTs). As in other hypocrealean fungi, gene contents and order were found to be conserved in the mt genome of O. sinensis, but the genome size was enlarged by longer intergenic regions and numerous introns. Intergenic and intronic regions were composed of abundant repetitive sequences usually associated with mobile elements. It is likely that intronic ncORFs, which encode RTs and HEs, may have contributed to the enlarged mt genome of O. sinensis.