Inhibition of prostaglandin E2 production by anti-inflammatory Hypericum perforatum extracts and constituents in RAW264.7 mouse macrophage cells
Inhibition of prostaglandin E2 production by anti-inflammatory Hypericum perforatum extracts and constituents in RAW264.7 mouse macrophage cells
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DOI：
10.1021/jf0710074
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发表时间：
2007-09-05
影响因子：
6.1
通讯作者：
Birt, Diane F.
中科院分区：
文献类型：
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作者：
Hammer, Kimberly D. P.;Hillwig, Matthew L.;Birt, Diane F.
贯叶金丝桃（Hypericum，perforatum，Hp）因其抗病毒、抗抑郁和细胞毒性而广为人知，但传统上Hp也被用于治疗炎症。在这项研究中，不同的Hp提取物和加入和Hp提取物中存在的成分的抗炎活性和细胞毒性的特点。与Hp的抗病毒活性相反，用所有Hp提取物观察到的抗炎活性是不依赖于光的。当纯成分进行测试，类黄酮，穗花杉双黄酮，贯叶金丝桃素，和光激活的pseudohypericin，显示抗炎活性，虽然在浓度一般高于Hp提取物中存在的量。在Hp提取物中以抑制前列腺素E-2（PGE（2））产生的浓度存在的成分是假金丝桃素和贯叶金丝桃素，这表明它们是与类黄酮沿着的主要抗炎成分，并且这些成分与其他未鉴定的化合物的相互作用对于Hp提取物的抗炎活性可能是重要的。
Hypericum, perforatum (Hp) is commonly known for its antiviral, antidepressant, and cytotoxic properties, but traditionally Hp was also used to treat inflammation. In this study, the anti-inflammatory activity and cytotoxicity of different Hp extractions and accessions and constituents present within Hp extracts were characterized. In contrast to the antiviral activity of Hp, the anti-inflammatory activity observed with all Hp extracts was light-independent. When pure constituents were tested, the flavonoids, amentoflavone, hyperforin, and light-activated pseudohypericin, displayed anti-inflammatory activity, albeit at concentrations generally higher than the amount present in the Hp extracts. Constituents that were present in the Hp extracts at concentrations that inhibited the production of prostaglandin E-2 (PGE(2)) were pseuclohypericin and hyperforin, suggesting that they are the primary anti-inflammatory constituents along with the flavonoids, and perhaps the interactions of these constituents and other unidentified compounds are important for the anti-inflammatory activity of the Hp extracts.