Dual-mode probe based on mesoporous silica coated gold nanorods for targeting cancer cells
Dual-mode probe based on mesoporous silica coated gold nanorods for targeting cancer cells
复制标题
基于介孔二氧化硅涂层金纳米棒的双模式探针，用于靶向癌细胞
DOI：
10.1016/j.bios.2010.11.032
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发表时间：
2011-02-15
影响因子：
12.6
通讯作者：
Cui, Yiping
中科院分区：
文献类型：
--
作者：
Wang, Zhuyuan;Zong, Shenfei;Cui, Yiping
基于介孔二氧化硅包覆的金纳米棒（MS-GNRs）首次制备了一种靶向癌细胞的双模式成像探针。在该探针中，荧光和表面增强拉曼散射（Sers）信号可以通过使用不同的激发波长独立地产生。为了研究探针的靶向性能，将叶酸（FA）缀合在MS-GNR的外表面上作为靶向配体，并且使用HeLa细胞作为模型癌细胞，因为它们过表达叶酸受体（FR）。通过荧光图像和Sers图谱，与游离FA竞争实验验证了内吞机制。MTT法检测结果表明，与无硅壳的GNRs相比，该探针的细胞毒性显著降低。与传统的成像探针相比，这种新型的纳米探针在活细胞中具有很大的多重成像潜力，可以很容易地通过使用荧光和Sers信号来实现。(C)2010爱思唯尔有限公司版权所有。
A dual-mode imaging probe for targeting cancer cells has been fabricated based on mesoporous silica coated gold nanorods (MS-GNRs) for the first time. In this probe, fluorescence and surface enhanced Raman scattering (SERS) signals can be generated independently by using different excitation wavelengths. To investigate the targeting performance of the probe, folic acid (FA) is conjugated on the outer surfaces of MS-GNRs as a targeting ligand and HeLa cells were used as model cancer cells because they overexpress folate receptors (FRs). The endocytosis mechanism was verified by competing experiments with free FA through both fluorescence images and SERS mappings. Moreover, the cytotoxicity of the probe was remarkably reduced in comparison with the GNRs without the silica shell as proved by the results of MTT assay. Compared with traditional imaging probes, this new type of nanoprobe has great potential for multiplexed imaging in living cells, which can be easily realized by using fluorescence and SERS signals. (C) 2010 Elsevier B.V. All rights reserved.