Development of inhibitor-directed enzyme prodrug therapy (IDEPT) for prostate cancer.
Development of inhibitor-directed enzyme prodrug therapy (IDEPT) for prostate cancer.
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DOI：
10.1021/bc500362n
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发表时间：
2014-10-15
影响因子：
4.7
通讯作者：
Black, Margaret E.
中科院分区：
文献类型：
--
作者：
Martin, Stacy E.;Ganguly, Tanushree;Munske, Gerhard R.;Fulton, Melody D.;Hopkins, Mark R.;Berkman, Clifford E.;Black, Margaret E.
Prostate cancer (PCa) is the second most common cause of cancer death among American men after lung cancer. Unfortunately, current therapies do not provide effective treatments for patients with advanced, metastatic, or hormone refractory disease. Therefore, we seek to generate therapeutic agents for a novel PCa treatment strategy by delivering a suicide enzyme (yCDtriple) to a cell membrane bound biomarker found on PCa cells (prostate-specific membrane antigen (PSMA)). This approach has resulted in a new PCa treatment strategy reported here as inhibitor-directed enzyme prodrug therapy (IDEPT). The therapeutic agents described were generated using a click chemistry reaction between the unnatural amino acid (p-azidophenylalanine (pAzF)) incorporated into yCDtriple and the dibenzylcyclooctyne moiety of our PSMA targeting agent (DBCO-PEG4-AH2-TG97). After characterization of the therapeutic agents, we demonstrate significant PCa cell killing of PSMA-positive cells. Importantly, we demonstrate that this click chemistry approach can be used to efficiently couple a therapeutic protein to a targeting agent and may be applicable to the ablation of other types of cancers and/or malignancies.
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影响因子：
56.9
作者：
Korkegian, A;Black, ME;Stoddard, BL
通讯作者：
Stoddard, BL
影响因子：
4.9
作者：
Kularatne, Sumith A.;Wang, Kevin;Low, Philip S.
通讯作者：
Low, Philip S.
影响因子：
15
作者：
Kim CH;Axup JY;Dubrovska A;Kazane SA;Hutchins BA;Wold ED;Smider VV;Schultz PG
通讯作者：
Schultz PG
影响因子：
3.8
作者：
Goerke, Aaron R.;Swartz, James R.
通讯作者：
Swartz, James R.
影响因子：
7.3
作者：
Kularatne, Sumith A.;Venkatesh, Chelvam;Low, Philip S.
通讯作者：
Low, Philip S.