Type 1/type 2 inflammatory cytokines correlate with olfactory function in patients with chronic rhinosinusitis
Type 1/type 2 inflammatory cytokines correlate with olfactory function in patients with chronic rhinosinusitis
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1型/2型炎症细胞因子与慢性鼻窦炎患者的嗅觉功能相关
DOI：
10.1016/j.amjoto.2020.102587
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发表时间：
2020-09-01
影响因子：
2.5
通讯作者：
Wei, Yongxiang
中科院分区：
文献类型：
--
作者：
Han, Xingyu;Wu, Dawei;Wei, Yongxiang
背景：慢性鼻窦炎（CRS）继发的嗅觉功能障碍与生活质量受损密切相关。亚洲 CRS 患者表现出独特的炎症特征，与欧洲和北美患者相比，2 型内型较少。本研究旨在探讨中国 CRS 患者炎症细胞因子的模式及其与嗅觉功能的关系。方法：机构审查委员会批准的前瞻性研究，其中 71 名 CRS 患者在鼻内窥镜手术前使用 Sniffin' Sticks 评估嗅觉功能。使用多重流式细胞珠测定 (CBA) 测量鼻粘液中的一组细胞因子和炎症介质，包括 1 型和 2 型炎症细胞因子。收集CRS患者的基线特征，并进行Spearman r统计，评估嗅觉功能与细胞因子和炎症介质的关系。结果：共71例CRS患者的鼻粘液样本，其中25例不伴有鼻息肉病的慢性鼻窦炎（CRSsNP）患者和46例慢性鼻窦炎伴鼻息肉病（CRSwNP）患者， 在这项研究中进行了评估。鼻粘液中 1 型炎症细胞因子 IFN-γ（干扰素-γ）、2 型炎症细胞因子包括 IL-4、IL-5 和 GM-CSF（粒细胞巨噬细胞集落刺激因子）以及抗炎细胞因子 IL-10 的水平与 CRS 患者的嗅觉功能呈显着负相关（r = - 0.308，p = 0.009； r = -0.250，p = 0.036； r = - 0.399，p = 0.001； r = - 0.269，p = 0.023； r = - 0.273，p = 0.021，分别）。在 CRSsNP 中，嗅觉功能与 1 型炎症细胞因子 TNF-α（肿瘤坏死因子-α）（r = - 0.637，p = 0.001）和 IL-10（r = - 0.468，p = 0.018）的水平呈负相关。然而，CRSwNP 的嗅觉功能与 2 型炎症细胞因子（包括 IL-4（r = - 0.303，p = 0.041）和 IL-5（r = -0.383，p = 0.009））呈负相关。 结论：1 型和 2 型炎症细胞因子可能与中国人 CRS 相关嗅觉功能障碍的发病有关。 人口。
Background: Olfactory dysfunction secondary to chronic rhinosinusitis (CRS) has been highly associated with impaired quality of life. Asian CRS patients showed a distinct inflammatory profile, with less type 2 endotype compared with European and North American. This study aimed to explore the pattern of the inflammatory cytokines in CRS patients from China and their association with olfactory function.Methods: Institutional review board-approved prospective study in which the olfactory function of 71 CRS patients was assessed with Sniffin' Sticks before the nasal endoscopic surgery. A set of cytokines and inflammatory mediators including type 1 and type 2 inflammatory cytokines were measured in nasal mucus by using a multiplex flow cytometric bead assay (CBA). Baseline characteristics in CRS patients were collected and the Spearman r statistic was performed to assess the association of olfactory function with cytokines and inflammatory mediators.Results: A total of 71 nasal mucus samples of CRS patients, including 25 chronic rhinosinusitis without nasal polyposis (CRSsNP) patients and 46 chronic rhinosinusitis with nasal polyposis (CRSwNP) patients, were evaluated in this study. The nasal mucus levels of type 1 inflammatory cytokine IFN-gamma (interferon-gamma), type 2 inflammatory cytokines including IL-4, IL-5 and GM-CSF (granulocyte-macrophage colony-stimulating factor) and anti-inflammatory cytokine IL-10 were significantly and inversely correlated with olfactory function in total patients with CRS (r = - 0.308, p = 0.009; r = -0.250, p = 0.036; r = - 0.399, p = 0.001; r = - 0.269, p = 0.023; r = - 0.273, p = 0.021, respectively). In CRSsNP, the olfactory function was inversely correlated with levels of type 1 inflammatory cytokine TNF-alpha (tumor necrosis factor-alpha) (r = - 0.637, p = 0.001) and IL-10 (r = - 0.468, p = 0.018). Nevertheless, the olfactory function in CRSwNP was inversely correlated with type 2 inflammatory cytokines including IL-4 (r = - 0.303, p = 0.041) and IL-5 (r = -0.383, p = 0.009).Conclusion: Both type 1 and type 2 inflammatory cytokines may contribute to the pathogenesis of CRS-associated olfactory dysfunction in the Chinese population.