Replication of single-stranded DNA templates by primase-polymerase complexes of the yeast, Saccharomyces cerevisiae.
Replication of single-stranded DNA templates by primase-polymerase complexes of the yeast, Saccharomyces cerevisiae.
复制标题
通过酿酒酵母的引物酶-聚合酶复合物复制单链 DNA 模板。
DOI：
10.1093/nar/16.14.6411
复制
发表时间：
1988
影响因子：
14.9
通讯作者：
Biswas,SB
中科院分区：
文献类型：
--
作者：
Biswas,EE;Biswas,SB
来自酿酒酵母的部分纯化的引物酶-聚合酶复合物能够高效地将单链环状噬菌体DNA复制成复制形式。引物酶-聚合酶复合物对单引物环状 ssDNA 以及缺口 DNA 表现出引物酶活性和聚合酶活性。此外，它还能够通过偶联的引物酶-聚合酶作用复制未引物的单链环状噬菌体 DNA。在 Biogel A-0.5m 过滤中，引物酶-聚合酶活性出现在空隙体积中，表明质量大于 500 千道尔顿。引物酶和各种引物酶-聚合酶复合物在单链DNA模板上合成独特的引物，引物的大小分布取决于DNA的结构和引物酶-聚合酶组装体的性质。
A partially purified primase-polymerase complex from the yeast,Saccharomycescerevisiaewas capable of replicating a single stranded circular phage DNA into a replicative form with high efficiency. The primase-polymerase complex exhibited primase activity and polymerase activity on singly primed circular ssDNA as well as on gapped DNA. In addition, it was able to replicate an unprimed, single-stranded, circular phage DNA through a coupled primase-polymerase action. On Biogel A-0.5m filtration the primase-polymerase activities appeared in the void volume, demonstrating a mass of greater than 500 kilodaltons. Primase and various primase-polymerase complexes synthesized unique primers on single stranded DNA templates and the size distribution of primers was dependent on the structure of the DNA and the nature of the primase-polymerase assembly.