Hemocompatibility of electrospun halloysite nanotube‐ and carbon nanotube‐doped composite poly(lactic‐co‐glycolic acid) nanofibers
Hemocompatibility of electrospun halloysite nanotube‐ and carbon nanotube‐doped composite poly(lactic‐co‐glycolic acid) nanofibers
复制标题
DOI：
10.1002/app.38054
复制
发表时间：
2013-03
影响因子：
3
通讯作者：
Yili Zhao;Shige Wang;Qingshan Guo;Mingwu Shen;Xiangyang Shi
中科院分区：
文献类型：
--
作者：
Yili Zhao;Shige Wang;Qingshan Guo;Mingwu Shen;Xiangyang Shi
纳米纤维支架或其他设备，如心血管或血液接触医疗设备的主要问题之一是它们的机械性能较弱，其表面缺乏血液相容性。本研究将埃洛石纳米管(HNTs)和碳纳米管(CNTs)复合到聚乳酸-乙醇酸共聚(PLGA)纳米纤维中，研究了不同掺杂量的复合纳米纤维的力学性能和血液相容性。用扫描电子显微镜和透射电子显微镜表征了掺杂纳米管在纳米纤维中的形貌和内部分布。利用材料试验机对电纺纳米纤维的力学性能进行了测试。通过溶血法和抗凝血法分别检测了复合纳米纤维的血液相容性。我们发现，掺杂的HNTs或CNTs以同轴的方式分布在纳米纤维中，HNTs或CNTs的加入并没有显著改变PLGA纳米纤维的形态。重要的是，在PLGA纳米纤维中掺入HNTs或CNTs显著提高了PLGA纳米纤维的力学性能，无论是否掺杂HNTs和CNTs，PLGA纳米纤维都表现出良好的抗凝性能，而对人红细胞的溶血作用可以忽略不计。由于HNTs和CNTs掺杂的PLGA复合纳米纤维具有良好的力学性能、良好的血液相容性和生物相容性，这些复合纳米纤维可以作为组织工程应用的治疗性人工组织/器官替代品。©2012威利期刊公司J.应用。波兰姆。《科学》，2013
One of the major problems of nanofiber scaffold or other devices like cardiovascular or blood-contacting medical devices is their weak mechanical properties and the lack of hemocompatibility of their surfaces. In this study, halloysite nanotubes (HNTs) and carbon nanotubes (CNTs) were incorporated within poly(lactic-co-glycolic acid) (PLGA) nanofibers and the mechanical property and hemocompatibility of both types of composite nanofibers with different doping levels were thoroughly investigated. The morphology and internal distribution of the doped nanotubes within the nanofibers were characterized using scanning electron microscopy and transmission electron microscopy. Mechanical properties of the electrospun nanofibers were tested using a material testing machine. The hemocompatibility of the composite nanofibers was examined through hemolytic and anticoagulant assay, respectively. We show that the doped HNTs or CNTs are distributed in the nanofibers with a coaxial manner and the incorporation of HNTs or CNTs does not significantly change the morphology of the PLGA nanofibers. Importantly, the incorporation of HNTs or CNTs within PLGA nanofibers significantly improves the mechanical property of PLGA nanofibers, and PLGA nanofibers with or without doping of the HNTs and CNTs display good anticoagulant property and negligible hemolytic effect to human red blood cells. With the enhanced mechanical property, great hemocompatibility, and previously demonstrated biocompatibility of both HNTs- and CNTs-doped composite PLGA nanofibers, these composite nanofibers may be used as therapeutic artificial tissue/organ substitutes for tissue engineering applications. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013