Natural history-guided omics reveals plant defensive chemistry against leafhopper pests
Natural history-guided omics reveals plant defensive chemistry against leafhopper pests
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DOI：
10.1126/science.abm2948
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发表时间：
2022-02-04
期刊：
影响因子：
56.9
通讯作者：
Li, Dapeng
中科院分区：
文献类型：
--
作者：
Bai, Yuechen;Yang, Caiqiong;Li, Dapeng
尽管人们对植物非寄主抗病性的研究很多，但对植食性动物的非寄主抗病性却知之甚少，尽管它在农业上很重要。假眼小绿叶蝉是严重的农业害虫，通过窃听茉莉酸（JA）介导的未知信号输出来识别寄主植物。正向和反向遗传学系的原生烟草植物进行了筛选，在原生栖息地与原生食草动物使用高通量基因组学，转录组学和代谢组学工具，以揭示小绿叶蝉引起的JA-JAZi模块。该模块诱导一种未表征的咖啡酰腐胺-绿叶挥发性化合物，由多酚氧化酶在迈克尔加成反应中催化，我们在体外重建;在作物植物中进行工程，其中它需要小檗碱桥酶样2（BBL 2）用于其合成;并显示它赋予叶蝉抗性。自然历史引导的正向遗传学揭示了一个保守的非寄主抗性机制，可用于作物保护。
Although much is known about plant traits that function in nonhost resistance against pathogens, little is known about nonhost resistance against herbivores, despite its agricultural importance. Empoasca leafhoppers, serious agricultural pests, identify host plants by eavesdropping on unknown outputs of jasmonate (JA)-mediated signaling. Forward- and reverse-genetics lines of a native tobacco plant were screened in native habitats with native herbivores using high-throughput genomic, transcriptomic, and metabolomic tools to reveal an Empoasca-elicited JA-JAZi module. This module induces an uncharacterized caffeoylputrescine-green leaf volatile compound, catalyzed by a polyphenol oxidase in a Michael addition reaction, which we reconstitute in vitro; engineer in crop plants, where it requires a berberine bridge enzyme-like 2 (BBL2) for its synthesis; and show that it confers resistance to leafhoppers. Natural history-guided forward genetics reveals a conserved nonhost resistance mechanism useful for crop protection.