Evaluation of Electronspun Silk Fibroin-Based Transplants Used for Facial Nerve Repair
Evaluation of Electronspun Silk Fibroin-Based Transplants Used for Facial Nerve Repair
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DOI：
10.1097/mao.0b013e31827b4bd4
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发表时间：
2013-02-01
影响因子：
2.1
通讯作者：
Lan, Qing
中科院分区：
文献类型：
--
作者：
Hu, Aijun;Zuo, Baoqi;Lan, Qing
目的：研究再生的静电纺丝丝素蛋白（SF）纳米纤维作为神经导管能否改善神经再生微环境，诱导Sprague-Dawley大鼠面神经再生。设计：制备静电纺丝SF纳米纤维桥接Sprague-Dawley大鼠面神经缺损5 mm。结果：SF-1000导管在体内具有良好的生物相容性，无明显的局部炎症反应和瘢痕形成。术后3个月，形态学和功能学观察均显示，自体神经移植物和电纺丝素膜移植物对周围神经损伤后的修复效果有积极的评价。结论：电纺丝素膜移植物能促进周围神经损伤后的再生，有望成为替代自体神经移植物的新型神经移植物。
Objective: To study whether regenerated electrospun silk fibroin (SF) nanofibers as nerve conduits could improve nerve regeneration microenvironment and induce the facial nerve regeneration of Sprague-Dawley rats.Design: Electrospun SF nanofibers were prepared to bridge a 5-mm facial nerve defect in Sprague-Dawley rats. Three months after implantation, a comprehensive morphologic and functional evaluation was performed by electrophysiology, histology, Fluorogold retrograde tracing, and transmission electron micrograph.Results: The SF nanofiber tube exhibited good biocompatibility in vivo, and no distinct regional inflammation response and scar formation was observed. After 3 months of operation, the morphologic and functional investigation has shown a positive evaluation on the nerve repair outcome elicited by SF nanofiber graft and autograft.Conclusion: Electrospun SF grafts could promote nerve regeneration after facial nerve injury and become a potential possibility of newly developed nerve grafts as an alternative of autografts to peripheral nerve regeneration.