Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved.
Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved.
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DOI：
10.2166/wst.2021.071
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发表时间：
2021-03
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影响因子：
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通讯作者：
W. Dai;Gao Chen;X. Bi;Huairong Zhong;Xueying Wang;S. Dong;D. Lv;Shulin Zhang;Dajuan Zhang;Na Wang
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作者：
W. Dai;Gao Chen;X. Bi;Huairong Zhong;Xueying Wang;S. Dong;D. Lv;Shulin Zhang;Dajuan Zhang;Na Wang
蓝藻在一段时间的资源剥夺后获得足够的资源时出现的快速超补偿生长可能有助于蓝藻水华的发生。研究了铜绿微囊藻（Microcystis aeruginosa）在光限制条件下的超补偿生长和丝氨酸/苏氨酸激酶（STK）基因表达的变化特征。结果表明，M.铜绿假单胞菌在光照恢复后2天表现出超补偿生长，在此期间生长的增加与光照强度呈负相关。光恢复后0.5 ~ 4 h，SPkD等STK基因表达显著上调（P < 0.05）。为探讨STK基因在超补偿生长中的作用，采用M.铜绿假单胞菌spkD在集胞藻中异源表达。转基因集胞藻表现出更大和更长的超补偿增长比野生型集胞藻后，光恢复。恢复光照24 h后，转基因集胞藻STK基因的相对表达量显著高于野生型集胞藻（P < 0.05）。微囊藻spkD的异源表达可能通过影响集胞藻STK基因的表达来刺激其超补偿生长。
The rapid overcompensatory growth that appears when cyanobacteria are supplied with adequate resources after a period of resource deprivation might contribute to the occurrence of cyanobacterial blooms. We investigated the changing characteristics of overcompensatory growth and serine/threonine kinase (STK) genes expression of cyanobacterium Microcystis aeruginosa in response to light limitation. The results showed M. aeruginosa exhibited overcompensatory growth for 2 days after light recovery, during which the increase in growth was inversely related to light intensity. Expression of STK genes, such as spkD, was upregulated significantly at 0.5-4 h after light recovery (P < 0.05). To investigate the function of STK genes in the overcompensatory growth, M. aeruginosa spkD was heterologously expressed in Synechocystis. Transgenic Synechocystis exhibited greater and longer overcompensatory growth than wild-type Synechocystis after light recovery. Relative expression levels of STK genes in transgenic Synechocystis were significantly higher than those in wild-type Synechocystis at 24 h of light recovery (P < 0.05). Heterologous expression of Microcystis spkD might stimulate overcompensatory growth of Synechocystis by affecting its STK gene expression.