PDR9 allelic variation and MYB63 modulate nutrient-dependent coumarin homeostasis in Arabidopsis
PDR9 allelic variation and MYB63 modulate nutrient-dependent coumarin homeostasis in Arabidopsis
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DOI：
10.1111/tpj.16678
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发表时间：
2024-02-15
期刊：
影响因子：
7.2
通讯作者：
Rouached，Hatem
中科院分区：
文献类型：
--
作者：
Deloose，Megan;Cho，Huikyong;Rouached，Hatem
植物根部将植物化学物质释放到土壤环境中，影响养分的有效性和吸收。拟南芥根部释放苯丙香豆素以应对铁 (Fe) 缺乏，可能会增强铁的吸收并改善植物健康。这种反应需要根部环境中有足够的磷（P）。尽管如此，在铁和磷的可用性不同的情况下影响香豆素生产的监管相互作用尚不清楚。通过全基因组关联研究，我们查明了 ABC 转运蛋白 G 家族成员 PDR9 在 Fe 和 P 联合缺乏的情况下对香豆素积累和运输（稳态）的影响。我们发现 PDR9 启动子的遗传变异以与香豆素产生相关的方式调节其表达。此外，我们发现 MYB63 转录因子通过调节香豆素合成酶 (COSY) 和阿魏酰辅酶 A 6'-羟化酶 1 (F6'H1) 的表达来控制专用香豆素的产生，同时在 Fe 和 P 联合缺乏的情况下通过 PDR9 基因协调分泌。这种综合方法阐明了香豆素反应机制中营养信号通路之间的复杂联系。
Plant roots release phytochemicals into the soil environment to influence nutrient availability and uptake.Arabidopsis thalianaroots release phenylpropanoid coumarins in response to iron (Fe) deficiency, likely to enhance Fe uptake and improve plant health. This response requires sufficient phosphorus (P) in the root environment. Nonetheless, the regulatory interplay influencing coumarin production under varying availabilities of Fe and P is not known. Through genome‐wide association studies, we have pinpointed the influence of the ABC transporter G family member, PDR9, on coumarin accumulation and trafficking (homeostasis) under combined Fe and P deficiency. We show that genetic variation in the promoter ofPDR9regulates its expression in a manner associated with coumarin production. Furthermore, we find that MYB63 transcription factor controls dedicated coumarin production by regulating bothCOUMARIN SYNTHASE(COSY) andFERULOYL‐CoA 6′‐HYDROXYLASE 1(F6′H1) expression while orchestrating secretion throughPDR9genes under Fe and P combined deficiency. This integrated approach illuminates the intricate connections between nutrient signaling pathways in coumarin response mechanisms.