LM23 is essential for spermatogenesis in Rattus norvegicus.
LM23 is essential for spermatogenesis in Rattus norvegicus.
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DOI：
10.2741/e81
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Mei-ling Liu;Yi-Ming Cheng;M. Jia
中科院分区：
文献类型：
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作者：
Mei-ling Liu;Yi-Ming Cheng;M. Jia
LM23基因是褐家鼠睾丸特异表达的基因。为了揭示LM23在睾丸中的功能，我们利用慢病毒介导的RNA干扰(RNAi)在体内以组织特异性的方式下调LM23的表达。将表达针对LM23的短发夹状RNA(ShRNA)的慢病毒载体显微注射到褐家鼠睾丸传出管内。与对照组相比，治疗组大鼠睾丸中LM23的表达明显下调。这些LM23-shRNA检测到的生殖细胞滞留在精母细胞阶段，并表现出凋亡增加和一些减数分裂基因的失调。这些结果证明了RNAi方法针对LM23的有效性，并揭示了LM23在睾丸中的表达在褐家鼠精子发生过程中的减数分裂中起关键作用。
LM23 is a gene with testis-specific expression in Rattus norvegicus. To reveal the function of LM23 in the testis, we used lentivirus-mediated RNA interference (RNAi) to knock down LM23 expression in a tissue-specific manner in vivo. A lentiviral vector expressing a short hairpin RNA (shRNA) targeting LM23 was microinjected into the efferent ducts of R. norvegicus testes. The expression of LM23 in the treated testes was significantly knocked down compared with controls. These LM23-shRNA testes contained germ cells arrested at the spermatocyte stage, and showed increased apoptosis and disregulation of some meiotic genes. The results demonstrate the validity of the RNAi approach for targeting LM23 and reveal that LM23 expression in the testis is crucial for meiosis during spermatogenesis in R. norvegicus.