STRAIN DIFFERENCES IN FINE SPECIFICITY OF MOUSE ANTIHAPTEN ANTIBODIES
STRAIN DIFFERENCES IN FINE SPECIFICITY OF MOUSE ANTIHAPTEN ANTIBODIES
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DOI：
10.1002/eji.1830030602
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发表时间：
1973-01-01
影响因子：
5.4
通讯作者：
MAKELA, O
中科院分区：
文献类型：
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作者：
IMANISHI, T;MAKELA, O
用偶联有鸡球蛋白的（4-羟基-3-硝基苯基）乙酰基（NP）免疫6个品系的小鼠。通过估计相关化合物（4-羟基-3，5-二硝基-苯基）乙酰基（NNP）、（5-溴-4-羟基-3-硝基苯基）乙酰基（NBrP）和（4-羟基-5-碘-3-硝基苯基）乙酰基（NIP）基团的相对亲和力来表征这些抗体的良好特异性。对血清和单个空斑形成细胞抗体进行这些测量。在这些菌株中可以识别出至少三种精细特异性模式。C57 BL/6产生7 S（IgG）抗NP，其对NIP的亲和力始终比对NP高3-10倍，对NNP和NBrP的亲和力比对NP高（不规则）。通过对血清和空斑形成细胞抗体进行亲和力测量，我们可以证明C57 BL/6小鼠产生的大多数7 S（IgG）抗NP在免疫早期和后期都是不规则的。此外，来自该菌株的抗体在免疫应答期间仅表现出亲和力的轻微变化，而其他菌株经历了明显的成熟（对NP的亲和力逐渐增加，并且NP和相关化合物之间的区分增加）C57 BL/6和CBA之间的9只F1杂交小鼠产生的抗NP抗体与C57 BL/6抗NP非常相似。
Mice of six strains were immunized with (4‐hydroxy‐3‐nitrophenyl)acetyl (NP) groups coupled to chicken globulin. The fine specificities of these antibodies were characterized by estimating relative affinities for related compounds, (4‐hydroxy‐3, 5‐dinitro‐phenyl)acetyl (NNP), (5‐bromo‐4‐hydroxy‐3‐nitrophenyl)acetyl (NBrP) and (4‐hydroxy‐5‐iodo‐3‐nitrophenyl)acetyl (NIP) groups. These measurements were carried out on both serum and single plaque‐forming cell antibodies. At least three patterns of fine specificity could be recognized among these strains.C57BL/6 produced 7 S (IgG) anti‐NP that consistently had 3–10 times higher affinity for NIP than for NP, and higher affinity for NNP and NBrP than for NP (heteroclitic). By doing affinity measurements on serum and plaque‐forming cell antibodies, we could demonstrate that the majority of the 7 S (IgG) anti‐NP produced by C57BL/6 mice was heteroclitic at both early and later stages of immunization. Moreover, the antibody from this strain exhibited only a slight change in affinity during the immune response, while the other strains underwent clearcut maturation (progressively increased affinity for NP and increased discrimination between NP and the related compound).Nine F1hybrid mice between C57BL/6 and CBA produced anti‐NP antibody that was very similar to C57BL/6 anti‐NP.