Associations between two single nucleotide polymorphisms of adiponectin gene and coronary artery diseases
Associations between two single nucleotide polymorphisms of adiponectin gene and coronary artery diseases
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DOI：
10.1507/endocrj.k06-020
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发表时间：
2006-10-01
影响因子：
2
通讯作者：
Park, Jung-Roe
中科院分区：
文献类型：
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作者：
Jung, Chan-Hee;Rhee, Eun-Jung;Park, Jung-Roe
脂联素是一种脂肪细胞分泌的蛋白质，已知具有抗动脉粥样硬化、抗炎和抗糖尿病的特性，在肥胖、2型糖尿病和冠状动脉疾病中其血清水平降低。已经进行了几项研究来调查脂联素遗传变异与肥胖、胰岛素抵抗和2型糖尿病的关系，但很少有研究与冠状动脉疾病的关系。因此，我们研究了脂联素基因的两个单核苷酸多态性（snp）， +45T > G和+276G > T与冠状动脉疾病（CAD）之间的关系。156名受试者（平均年龄57.4岁）因胸痛而行冠状动脉造影。采用Taqman聚合酶链反应（PCR）法对脂联素基因的两个snp进行基因分型。冠心病的存在被定义为冠状动脉直径缩小50%。156名受试者中，SNP +276G > G中G等位基因频率为0.683，T等位基因频率为0.317;SNP +45T > G中T等位基因频率为0.705，G等位基因频率为0.295，均符合Hardy-Weinberg平衡。脂联素基因SNP276和SNP45与冠心病无相关性（p = 0.954, p = 0.843）。此外，在冠心病的严重程度和snp之间没有观察到显著的关联（p=0.571, p=0.955）。我们的研究表明，脂联素基因SNP +276G > T和+45T > G与CAD的存在无关。需要进一步的研究来证实SNP 276G > T和45T > G在CAD发展中的作用。
Adiponectin, an adipocyte-secreted protein, is known to have anti-atherogenic, anti-inflammatory and antidiabetic properties and its serum levels are decreased in obesity, type 2 diabetes, and coronary artery disease. Several studies have been performed to investigate the association of genetic variations in the adiponectin with obesity, insulin resistance, and type 2 diabetes, but few studies were performed in association with coronary artery disease. Therefore we examined the associations between two single nucleotide polymorphisms (SNPs), +45T > G and +276G > T of the adiponectin gene, and coronary artery diseases (CAD). One hundred and fifty six subjects (mean age 57.4 yrs) were enrolled in which coronary angiograms were performed due to chest pain. Genotypings were done for two SNPs in the adiponectin gene by Taqman polymerase chain reaction (PCR) method. The presence of CAD was defined as a > 50% reduction of coronary artery diameter. Among 156 subjects, the allele frequencies were 0.683 for G allele and 0.317 for T allele in SNP +276G > T and 0.705 for T allele and 0.295 for G allele in SNP +45T > G. Both genotypes were in compliance with Hardy-Weinberg equilibrium. No association with the presence of CAD was observed for adiponectin gene SNP276 and SNP45 (p = 0.954, p = 0.843). Also, no significant association was observed between the severity of CAD and either SNPs(p=0.571, p=0.955). Our study showed that SNP +276G > T and +45T > G in adiponectin gene were not associated with the presence of CAD. Further studies will be necessary to confirm the role of SNP 276G > T and 45T > G in the development of CAD.