Research on the correlation between platelet gelsolin and blood-stasis syndrome of coronary heart disease
Research on the correlation between platelet gelsolin and blood-stasis syndrome of coronary heart disease
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血小板凝溶胶蛋白与冠心病血瘀证相关性研究
DOI：
10.1007/s11655-011-0814-z
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发表时间：
2011-08-01
影响因子：
2.9
通讯作者：
Chen Ke-ji
中科院分区：
文献类型：
--
作者：
Liu Yue;Yin Hui-jun;Chen Ke-ji
目的研究凝胶素在人血小板和血浆中的分布及其与冠心病血瘀证的关系。方法选择冠心病血瘀证患者60例，其中血瘀组30例，非血瘀组30例，健康对照组30例。辨证分型以临床症状和体征为依据。采用酶联免疫吸附试验(ELISA)测定富血小板血浆(PRP)、贫血小板血浆(PPP)、丝状肌动蛋白(F-actin)和组特异性成分球蛋白(GC-球蛋白)中明胶蛋白的浓度。结果与对照组比较，血瘀证组PRP中明胶蛋白显著升高(P<0.01)，血瘀证组和非血瘀证组PPP中明胶蛋白含量显著降低(P<0.05)，血瘀证组和非血瘀证组患者血小板CD62P、[Ca~(2+)]i、F-肌动蛋白、GC-球蛋白显著升高(P<；0.01)。与非血瘀证组比较，血瘀证组PRP中明胶蛋白浓度显著升高(P<；0.01)，血瘀证组血小板[Ca~(2+)]i显著升高(P&t；0.01)，而血瘀证组F-肌动蛋白和GC-球蛋白无统计学差异(P>；0.05)。结论冠心病血瘀证患者PRP中明胶蛋白浓度升高，并伴有血小板[Ca~(2+)]i升高，而PPP中明胶蛋白显著降低。我们推测血浆明胶蛋白可能清除循环中的F-肌动蛋白，从而导致血浆明胶蛋白的显著耗竭。在CHD血瘀证的发病过程中，除血小板钙内流增加外，可能还导致了血小板明胶蛋白的异常表达。因此，血小板明胶蛋白可能成为冠心病血瘀证新的潜在生物标志物和治疗靶点。
ObjectiveTo study the distribution of gelsolin in human platelet and plasma, and the association with blood-stasis syndrome (BSS) of coronary heart disease (CHD).MethodsSixty patients with CHD (30 in BSS group and 30 in non-BSS group) and 30 healthy subjects (control group) were included in this study. The classification of the syndrome was based on clinical symptoms and signs. Gelsolin concentration in platelet rich plasma (PRP), platelet poor plasma (PPP), filamentous actin (F-actin) and group-specific component globulin (Gc-globulin) of PPP were determined by enzyme-linked immunosorbent assay (ELISA). The fluorescence intensity of CD62p and cytoplasmic calcium ([Ca2+]i) in human platelets of patients and healthy persons was measured with flow cytometry.ResultsCompared with the control group, gelsolin in PRP of the BSS group increased significantly (P<0.01), while that in PPP of the BSS and non-BSS groups decreased markedly (P<0.05), the CD62p, [Ca2+]iof platelet, F-actin, and Gc-globulin of the BSS and non-BSS groups increased significantly (P<0.01). Compared with the non-BSS group, the gelsolin concentration in PRP of BSS group increased significantly (P<0.01), the [Ca2+]iof platelet of the BSS group increased markedly (P<0.01), while the F-actin and Gc-globulin of the BSS group had no statistical defference (P>0.05).ConclusionsGelsolin concentration in PRP was increased and accompanied by the elevated [Ca2+]iof platelet in CHD with BSS, while gelsolin in PPP were lowered markedly. We speculate that plasma gelsolin may clear F-actin from circulation, thus resulting in depletion of plasma gelsolin significantly. This, in addition to the increased calcium influx of platelets, may lead to the gelsolin abnormal expression on platelets during the process of BSS in CHD. Therefore, platelet gelsolin may serve as a new potential biomarker and a therapeutic target of BSS in CHD.